Document 0124
 DOCN  M9590124
 TI    Chromatography in DNA radiolabeling: hands-off automation using a
       robotic workstation.
 DT    9509
 AU    Mischiati C; Feriotto G; Fiorentino D; Gambari R; Department of
       Biochemistry and Molecular Biology, Ferrara; University, Italy.
 SO    J Chromatogr B Biomed Appl. 1995 Feb 17;664(2):303-10. Unique Identifier
       : AIDSLINE MED/95299657
 AB    The experiments described in the present paper were performed in order
       to determine whether the Biomek-1000 (Beckman Instruments, Fullerton,
       CA, USA) automated laboratory workstation can be used in a fully
       automated DNA labeling method followed by automated gravity-driven size
       exclusion purification of molecular probes. To this aim, we performed
       random oligodeoxyribonucleotide priming of a HIV-1 LTR probe that was
       used for molecular hybridization to Southern blotted polymerase chain
       reaction products. The results obtained demonstrate that the
       automatically labeled probe can be efficiently purified by automated and
       gravity-driven Sephadex G-50 chromatography, without any major changes
       in hybridization property. This robotic methodology can be used in
       several procedures employing radioisotope labeling.
 DE    Base Sequence  Blotting, Southern  Cell Line  Chromatography, Gel
       DNA/*CHEMISTRY  DNA Probes  Human  HIV-1/GENETICS  HLA-DR
       Antigens/GENETICS  In Situ Hybridization  Isotope
       Labeling/*INSTRUMENTATION  Molecular Sequence Data  Polymerase Chain
       Reaction  Robotics  Support, Non-U.S. Gov't  T-Lymphocytes/METABOLISM
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).