Document 0133
 DOCN  M9590133
 TI    Interleukin-10 is an autocrine growth factor for acquired
       immunodeficiency syndrome-related B-cell lymphoma.
 DT    9509
 AU    Masood R; Zhang Y; Bond MW; Scadden DT; Moudgil T; Law RE; Kaplan MH;
       Jung B; Espina BM; Lunardi-Iskandar Y; et al; Department of Medicine,
       University of Southern California, Norris; Cancer Hospital and Research
       Institute, Los Angeles 90033, USA.
 SO    Blood. 1995 Jun 15;85(12):3423-30. Unique Identifier : AIDSLINE
       MED/95299125
 AB    Interleukin-10 (IL-10) is an acid-sensitive protein of 35 kD that has
       pleiotropic effects including inhibition of cytotoxic T-cell response,
       induction of major histocompatibility complex type II in B lymphocytes,
       induction of B-cell growth and differentiation, and autocrine growth
       factor activity in monocytes. We and others have shown that IL-10 is
       produced spontaneously by blood mononuclear cells from human
       immunodeficiency virus-seropositive patients. In an attempt to ascertain
       the potential role of IL-10 in acquired immunodeficiency syndrome
       (AIDS)-related B-cell lymphoma, we evaluated the expression of human
       IL-10 in both tumor-derived B-cell lines and primary tumor cells.
       Expression of human IL-10 (hIL-10) mRNA and protein was detected in four
       of five cell lines examined. An IL-10 antisense oligonucleotide
       inhibited IL-10 mRNA expression and IL-10 protein production. The
       proliferation of all B-cell lines was inhibited by an antisense
       oligonucleotide in a dose-dependent manner that was abrogated by the
       addition of recombinant hIL-10 protein. No effect of antisense
       oligonucleotide was observed in the B-cell line not producing hIL-10.
       Evaluation of primary tumor cells from patients with AIDS-lymphoma cells
       showed similar production and response to IL-10. These data suggest an
       autocrine growth mechanism for IL-10 in AIDS-related lymphoma cells and
       that IL-10 may be important in its pathogenesis.
 DE    B-Lymphocytes/METABOLISM/PATHOLOGY  Base Sequence  Cell Division/DRUG
       EFFECTS  Human  Interleukin-10/*BIOSYNTHESIS  Lymphoma,
       AIDS-Related/*METABOLISM/PATHOLOGY  Molecular Sequence Data
       Oligonucleotides, Antisense/PHARMACOLOGY  Recombinant
       Proteins/PHARMACOLOGY  RNA, Messenger/ANTAGONISTS & INHIB/BIOSYNTHESIS
       Support, U.S. Gov't, P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).