Document 0136
 DOCN  M9590136
 TI    Humoral response to Mycobacterium tuberculosis-specific antigens in
       African tuberculosis patients with high prevalence of human
       immunodeficiency virus infection.
 DT    9509
 AU    Thybo S; Richter C; Wachmann H; Maselle SY; Mwakyusa DH; Mtoni I;
       Andersen AB; Department of Mycobacteria, Statens Seruminstitut,
       Copenhagen,; Denmark.
 SO    Tuber Lung Dis. 1995 Apr;76(2):149-55. Unique Identifier : AIDSLINE
       MED/95299092
 AB    SETTING: The applicability of serodiagnosis of tuberculosis using
       Mycobacterium tuberculosis-complex-specific antigens in a Tanzanian
       population with high prevalence of HIV. OBJECTIVE: This study was
       performed to evaluate the usefulness, sensitivity and specificity of
       serology using M. tuberculosis-specific antigens in the diagnosis of
       tuberculosis in patients with and without HIV co-infection. DESIGN:
       Patients with proven pulmonary and extrapulmonary tuberculosis at a
       major referral centre in Tanzania were enrolled in the study. The
       control group consisted of patients without a history of previous
       tuberculosis admitted to the trauma ward and of healthy volunteers. Sera
       were analysed by an enzyme linked immunoassay (ELISA) using two M.
       tuberculosis specific proteins as antigen: the 38 kDa protein [3T] and a
       17 kDa protein. In addition was recorded presence or absence of BCG scar
       and tuberculin sensitivity and the sera were tested for HIV and analysed
       for beta-2-microglobulin content. RESULT: Sensitivity and specificity
       were markedly reduced in tuberculosis patients with HIV co-infection
       compared to patients without this disease (73% and 70% versus 52% and
       50% respectively). CONCLUSION: Serology for diagnosis of tuberculosis is
       not feasible in an HIV endemic region.
 DE    Antibodies, Bacterial/*BIOSYNTHESIS  Antigenic Determinants/*IMMUNOLOGY
       Enzyme-Linked Immunosorbent Assay  Human  HIV Infections/*COMPLICATIONS
       Mycobacterium tuberculosis/*IMMUNOLOGY  Sensitivity and Specificity
       Support, Non-U.S. Gov't  Tuberculin Test
       Tuberculosis/COMPLICATIONS/DIAGNOSIS/*IMMUNOLOGY  JOURNAL ARTICLE

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