Document 0139
 DOCN  M9590139
 TI    Early infection of interdigitating dendritic cells in the pig lymph node
       with African swine fever viruses of high and low virulence:
       immunohistochemical and ultrastructural studies.
 DT    9509
 AU    Gregg DA; Mebus CA; Schlafer DH; Foreign Animal Disease Diagnostic
       Laboratory, National Veterinary; Services Laboratories, APHIS, USDA,
       Greenport, NY 11944, USA.
 SO    J Vet Diagn Invest. 1995 Jan;7(1):23-30. Unique Identifier : AIDSLINE
       MED/95298930
 AB    The role of interdigitating dendritic cells (IDCs) in the early
       pathogenesis of African swine fever (ASF) was investigated using
       mandibular lymphoid tissue from normal pigs and pigs inoculated
       oronasally with highly virulent Lisbon 60 (L-60) and moderately virulent
       Dominican Republic 1979 (DR-2) ASF virus (ASFV) isolates.
       Paraffin-embedded tissue sections were immunostained for ASFV antigen
       and S-100 protein, a marker of IDCs, using an avidin-biotin alkaline
       phosphatase procedure. Swine IDCs were identified morphologically by
       light microscopy, electron microscopy, and S-100 immunostaining.
       Infection with ASFV caused a marked reduction in S-100 staining by 3
       days postinfection (DPI) that persisted through 14 DPI. Early ASFV
       infection of IDCs was demonstrated at 3 DPI by double
       immunohistochemical staining of cryosections and by transmission
       electron microscopy. These results support the hypothesis that the
       failure of a humoral immune response to virulent ASFV may be due to a
       primary infection of IDCs and the inability of IDCs to initiate an
       immune response. Infection of IDCs has also been demonstrated with human
       immunodeficiency virus (HIV-1), and these infections have some aspects
       in common.
 DE    African Swine Fever Virus/*ISOLATION & PURIF/*PATHOGENICITY  Animal
       Biotin  Comparative Study  Dendritic
       Cells/CYTOLOGY/ULTRASTRUCTURE/*VIROLOGY  Female  Immunohistochemistry
       Lymph Nodes/CYTOLOGY/ULTRASTRUCTURE/*VIROLOGY  Male  Microscopy,
       Electron  Reference Values  Swine  Virulence  JOURNAL ARTICLE

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