Document 0167
 DOCN  M9590167
 TI    Heat shock protein-induced T-lymphocyte propagation from endomyocardial
       biopsies in heart transplantation.
 DT    9509
 AU    Moliterno R; Woan M; Bentlejewski C; Qian J; Zeevi A; Pham S; Griffith
       BP; Duquesnoy RJ; Division of Transplantation Pathology, University of
       Pittsburgh; Medical Center, PA 15261, USA.
 SO    J Heart Lung Transplant. 1995 Mar-Apr;14(2):329-37. Unique Identifier :
       AIDSLINE MED/95298818
 AB    BACKGROUND: Recent studies have shown that heat shock proteins can be
       recognized by T cells during various immunologically mediated
       inflammatory processes. Injurious stimuli to cells induce an increased
       production of heat shock proteins which could lead to their cell surface
       expression and subsequent recognition by the immune system. We have
       postulated that allograft infiltrating cells may recognize heat shock
       proteins, especially during rejection. METHODS: This hypothesis was
       tested by incubating heart transplant biopsy specimens from 89 heart
       transplant recipients with soluble Mycobacterium tuberculosis extracts,
       a source of heat shock proteins or recombinant mycobacterial heat shock
       protein 65. T cell phenotypes were determined by flow cytometry.
       RESULTS: Mycobacterium tuberculosis extract can induce lymphocyte
       propagation from heart transplant biopsy specimens especially during
       rejection. A highly significant correlation was seen between
       Mycobacterium tuberculosis extract and interleukin-2-induced lymphocyte
       growth and an accelerated growth was seen for cultures incubated with
       Mycobacterium tuberculosis extract + interleukin-2. A second series of
       experiments has also shown the propagation of lymphocytes induced by
       recombinant mycobacterial heat shock protein-65. T-cell phenotype
       analysis of biopsy propagated lymphocytes has shown higher frequencies
       of CD8 cells in Mycobacterium tuberculosis extract and heat shock
       protein-65 propagated lymphocytes from early posttransplantation biopsy
       specimens, whereas, later on, most cultures showed a predominance of CD4
       cells. T-cell receptor gamma delta cells were more frequently found in
       biopsy-derived lymphocyte cultures from long-term survivors, especially
       after propagation with Mycobacterium tuberculosis extract and heat shock
       protein-65. These gamma delta cells expressed primarily the delta 1
       rather than the gamma 9 phenotype. CONCLUSIONS: These observations
       provide first evidence for the presence of heat shock protein-reactive
       lymphocytes in cellular infiltrates of transplants undergoing rejection.
 DE    Adult  Cells, Cultured  CD4-Positive T-Lymphocytes/PHYSIOLOGY
       CD8-Positive T-Lymphocytes/PHYSIOLOGY  Endocardium/*PATHOLOGY  Flow
       Cytometry  Heart Transplantation/IMMUNOLOGY/*PATHOLOGY  Heat-Shock
       Proteins/*PHARMACOLOGY  Human  Immunophenotyping  In Vitro
       Interleukin-2/PHARMACOLOGY  Mycobacterium tuberculosis
       Myocardium/*PATHOLOGY  Receptors, Antigen, T-Cell,
       gamma-delta/PHYSIOLOGY  Recombinant Proteins/PHARMACOLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/IMMUNOLOGY/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).