Document 0272
 DOCN  M9590272
 TI    Molecular analysis of inhibition of HIV production by interleukin 10 in
       primary human monocytes derived macrophages.
 DT    9509
 AU    Naif H; Chang C; Ho-Shon M; Li S; Cunningham AL; Department of Virology,
       National Centre for HIV Virology; Research, Westmead, NSW.
 SO    Annu Conf Australas Soc HIV Med. 1994 Nov 3-6;6:250 (unnumbered poster).
       Unique Identifier : AIDSLINE ASHM6/95291827
 AB    Monocytes/macrophages play a major role in the pathogenesis of HIV
       infection particularly in the asymptomatic state of the disease. The
       mechanisms that regulate HIV expression during different disease stages
       are not well understood. Cytokines, as immune regulators, may contribute
       to the clinical latency and influence HIV expression. We have found that
       IL-10 inhibits HIV-1Ba-L and three different clinical isolates in
       primary purified human monocytes. Viral production in cultured monocytes
       was measured by p24 antigen and mRNA expression analysed by Northern
       blot hybridisation. Effect of IL-10 on viral entry was studied by
       semi-quantitative PCR measuring the very early stages of reverse
       transcription and DNA synthesis at different time intervals after
       infection. Inhibition of HIV replication caused by IL-10 was evident
       from all three methods used to measure different stages of HIV
       replication. Our data suggests that one of the possible mechanisms of
       IL-10 suppresses HIV replication irrespective of whether TNF-a (known to
       upregulate HIV expression) is added before or after IL-10. However, the
       length of macrophages exposure to IL-10 is important. Another important
       action of IL-10 is its downregulatory effect on cellular RNAs
       (house-keeping genes), such as G3PDH, transferrin receptor, and 28S
       ribosomal RNA. IL-10 effect on monocytes differentiation was also
       investigated.
 DE    Cells, Cultured  Dose-Response Relationship, Drug  Human  HIV/*DRUG
       EFFECTS/GENETICS  Interleukin-10/*PHARMACOLOGY  Macrophages/*VIROLOGY
       Monocytes/*VIROLOGY  Virus Replication/*DRUG EFFECTS/GENETICS  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).