Document 0274
 DOCN  M9590274
 TI    The effect of GM-CSF on HIV-1 infection of monocyte-macrophages.
 DT    9509
 AU    Maerz A; Lopez A; Crowe S; Macfarlane Burnet Centre for Medical
       Research, Fairfield, VIC.
 SO    Annu Conf Australas Soc HIV Med. 1994 Nov 3-6;6:248 (unnumbered poster).
       Unique Identifier : AIDSLINE ASHM6/95291825
 AB    AIM: Published data suggest that GM-CSF increases HIV replication in
       monocyte-derived cell lines (Ref.1) and primary monocyte-derived
       macrophages (2). The objective of this study was to further investigate
       the effects of GM-CSF on monocyte-derived-macrophages infected with
       HIV-1 in vitro. METHODS: Monocytes isolated by density gradient
       centrifugation and plastic adherence were cultured in suspension for 5
       days. The cells were infected with HIV for 2 hours, then washed and
       cultured adherent for 10 days in the presence or absence recombinant
       human GM-CSF derived from both yeast (Genzyme) and E. coli expression
       systems. All experiments were performed with rigorous exclusion of LPS
       contamination. Infection was determined p24 EIA and flow cytometry. HIV
       cDNA was quantitated by PCR using the primer pair SK38/39, which detects
       almost full-length transcripts. RESULTS: GM-CSF, from both sources,
       inhibited HIV infection of macrophages in a dose dependent response.
       Preliminary results indicate that the block in viral replication is
       occurring after the reverse transcription step.
 DE    Cell Line  Dose-Response Relationship, Drug  Granulocyte-Macrophage
       Colony-Stimulating Factor/*PHARMACOLOGY  Human  HIV-1/*DRUG EFFECTS
       Macrophages/*VIROLOGY  Monocytes/*VIROLOGY  Virus Replication/DRUG
       EFFECTS  MEETING ABSTRACT

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