Document 0275
 DOCN  M9590275
 TI    The induction of in vivo superinfection and recombination using feline
       immunodeficiency virus as the model.
 DT    9509
 AU    Kyaw-Tanner MT; Greene WK; Park HS; Robinson WF; Department of
       Veterinary Pathology, University of Queensland, St.; Lucia, Brisbane.
 SO    Annu Conf Australas Soc HIV Med. 1994 Nov 3-6;6:247 (unnumbered poster).
       Unique Identifier : AIDSLINE ASHM6/95291824
 AB    FIV is a lentivirus which shares many characteristics with HIV-1, and is
       considered appropriate for use as a model of HIV infection. We
       investigated the hypothesis that under certain conditions, in vivo
       superinfection of cats with FIV, may occur. One FIV isolate (T91) was
       used to inoculate three FIV and FeLV-free cats. Blood from an
       FIV-infected cat (N), which contained two variants and differed from T91
       by at least 5% in nucleotide sequence in the env gene, was inoculated
       into a fourth cat. A fifth cat was inoculated simultaneously with both
       T91 and blood from N. After 22 weeks, two of the three cats initially
       infected with T91 were challenged with blood from N. At 30 weeks
       following initial infection, peripheral blood mononuclear cells were
       obtained from all cats, DNA extracted, and a segment of the env gene was
       PCR amplified, cloned and sequenced. Nucleotide sequence analysis of the
       cloned PCR product showed that virus isolates used for initial infection
       were recovered from cats not challenged with a second isolate. Challenge
       of cats with the blood of N following initial infection with T91
       resulted in superinfection occurring in one cat and recombination
       occurring in the other. This suggests that the immune response directed
       against the original isolate failed to protect against infection with
       the second (challenge) isolate. Manipulation of this system provides an
       opportunity to identify factors that determine the presence or absence
       of in vivo superinfection.
 DE    Animal  Cats  Feline Acquired Immunodeficiency
       Syndrome/IMMUNOLOGY/*VIROLOGY  Gene Expression Regulation,
       Viral/PHYSIOLOGY  Genes, env/GENETICS/IMMUNOLOGY  Immunodeficiency
       Virus, Feline/GENETICS/IMMUNOLOGY/*PATHOGENICITY  Immunologic
       Memory/IMMUNOLOGY  Monocytes/IMMUNOLOGY/VIROLOGY  Polymerase Chain
       Reaction  Superinfection/IMMUNOLOGY/*VIROLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).