Document 0282
 DOCN  M9590282
 TI    NEF protein of HIV-1 is a positive regulator of virus replication.
 DT    9509
 AU    Arunagiri CK; Peden K; Sankovich S; McPhee D; Azad A; Biomolecular
       Research Institute, Parkville.
 SO    Annu Conf Australas Soc HIV Med. 1994 Nov 3-6;6:240 (unnumbered poster).
       Unique Identifier : AIDSLINE ASHM6/95291817
 AB    An investigation into the functions of Nef on HIV-1 replication was
       undertaken. Two molecular clones of HIV-1 viz.LAI and pNL 4.3 were
       selected and three mutant clones of nef were constructed. Nef mutants
       from LAI were constructed by frame shift mutation at amino acids 33 and
       84 respectively and pNL 4.3 clone was constructed by point mutation of
       both initiating methionines. The mutant clones were assayed for their
       ability to replicate in primary human CD4 cells at different
       multiplicity of infections (MOI). We have also assayed for the
       expression of cell surface molecules by flow cytometry when equivalent
       moi were used. The results showed that Nef positively influenced the
       virus replication at low moi and N-terminus Nef is involved in the
       downmodulation CD4 and IL-2 receptors.
 DE    Antigens, CD4/GENETICS  Cloning, Molecular  Down-Regulation
       (Physiology)/GENETICS  Flow Cytometry  Gene Expression Regulation,
       Viral/PHYSIOLOGY  Genes, nef/*GENETICS  Human  HIV-1/*GENETICS  Point
       Mutation  Receptors, Interleukin-2/GENETICS  Virus Replication/*GENETICS
       MEETING ABSTRACT

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