Document 0290
 DOCN  M9590290
 TI    A novel Sp-1 site in the HIV-1 LTR.
 DT    9509
 AU    Peeters A; Deacon N; AIDS Molecular Biology Lab, NCHVR, Macfarlane
       Burnet Centre for; Medical Research, Fairfield, Vic.
 SO    Annu Conf Australas Soc HIV Med. 1994 Nov 3-6;6:219 (unnumbered
       abstract). Unique Identifier : AIDSLINE ASHM6/95291809
 AB    Transcription of HIV-1 is primarily regulated through the U3 region of
       the LTR. Immediately 5' from the transcriptional start site are three
       Sp-1 sites which are responsible for basal transcription and two NF-kB
       sites which are primarily responsible for activated transcription from
       the LTR. To investigate other proteins involved in transcriptional
       regulation of HIV-1 the LTR was divided into 13 doublestranded
       overlapping oligonucleotides and these were used to screen for further
       protein-DNA interactions. Three novel, specific protein-DNA interactions
       with the 5' end of the HIV-1 LTR have been observed using a 34 base pair
       oligonucleotide encompassing the HXB2R region n.t.-421 to 454 (Oligo 13)
       in mobility shift assays with MT-2 nuclear extracts. The protein sizes
       were approximately 25, 50 and 95kd by southwestern analysis. One of the
       Oligo 13 mobility shift interactions was competable by a doublestranded
       oligonucleotide encompassing the region of the HIV-1 LTR containing the
       previously characterized SP-1 sites (HXB2R n.t. -36 to -75) and a
       consensus Sp-1 site oligonucleotide. This interaction was supershifted
       in a mobility shift assay by a polyclonal anti Sp-1 antibody. Sp-1
       exists as a 95 or 105 kd protein and purified Sp-1 binds to oligo 13 in
       a specific manner. The affinity of the Sp-1:HIV-1 site and the
       SP-1:consensus SP-1 site interactions have been compared. Functional
       studies indicate a degree of transcriptional importance for this region
       of the HIV-1 LTR.
 DE    Cell Line  Gene Expression Regulation, Viral/PHYSIOLOGY  Human  HIV Long
       Terminal Repeat/*GENETICS  HIV-1/*GENETICS  Transcription Factor,
       Sp1/*GENETICS  Virus Integration/GENETICS  Virus Replication/GENETICS
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).