Document 0346
 DOCN  M9590346
 TI    Interleukin 10 inhibits replication in monocytes and macrophages.
 DT    9509
 AU    Chang J; Naif H; Li S; Fear W; Ho-Shon M; Kesson A; Cunningham AL;
       Westmead Hospital, NSW.
 SO    Annu Conf Australas Soc HIV Med. 1994 Nov 3-6;6:151 (unnumbered
       abstract). Unique Identifier : AIDSLINE ASHM6/95291753
 AB    Recent studies have shown that endogenous interleukin 10 (IL-10)
       produced either by IFN-gamma or LPS stimulated human monocytes, strongly
       inhibited the production of many proimflammatory cytokines at the
       transcriptional level. On the basis of marked immunoregulatory effects
       of IL-10 on monocytes, we investigated the biological responses of HIV
       infected monocytes, monocyte-derived-macrophages (MDM) and placental
       macrophages (PM) to exogenous IL-10. We measured the levels of HIV
       replication by extracellular p24 antigen and by intracellular HIV mRNA.
       IL-10 inhibited HIV replication in a concentration dependent manner when
       incubated with monocytes, MDM, PM prior to HIV inoculation. In monocytes
       and macrophages, IL-10 repeatedly had no significant effect on HIV
       replication when added after the infection. A minimum of 12-48 hours of
       pre-incubation with IL-10 was required for this inhibitory effect. The
       specificity of IL-10 effect was shown by reversal of inhibition
       following incubation with neutralizing antibody. The mechanism of this
       inhibition appeared to be indirect as IL-10 down-regulate many genes at
       te transcriptional level of uninfected monocytes, including
       house-keeping genes such as the gluceraldehyde-3-phosphate dehydrogenase
       and (28s) ribosomal RNA. IL-10 down-regulated the surface expression of
       HLA-DR on monocytes. In contrast, IL-4 had no effect and IL-10 together
       with IL-4 had a combined effect where they down regulated HLA-DR but not
       as much as IL-10 alone. Neither IL-10 nor IL-4 had any effect on the CD4
       expression on monocytes. These data indicate that IL-10 is a potent
       inhibitor of HIV replication in monocytes and macrophages but only when
       it is added prior to HIV infection.
 DE    Cells, Cultured  Human  HIV/*IMMUNOLOGY/PATHOGENICITY
       Interleukin-10/*PHYSIOLOGY  Macrophages/*IMMUNOLOGY/VIROLOGY
       Monocytes/*IMMUNOLOGY/VIROLOGY  Virus Replication/*IMMUNOLOGY  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).