Document 0390
 DOCN  M9590390
 TI    HIV-1 gp120 produces DNA fragmentation in the cerebral cortex of rat.
 DT    9509
 AU    Bagetta G; Corasaniti MT; Berliocchi L; Navarra M; Finazzi-Agro A;
       Nistico G; Department of Neuroscience, University of Cagliari, Italy.
 SO    Biochem Biophys Res Commun. 1995 Jun 6;211(1):130-6. Unique Identifier :
       AIDSLINE MED/95298012
 AB    In the present experiments we have used morphological techniques to
       study the neuropathological profile of the brain of rats after
       intracerebroventricular (i.c.v.) injection of recombinant HIV-1 gp 120.
       Using brain cryostat sections (10 microns) from rats treated with a
       single, daily dose of gp120 (100 ng/rat) given for 7 and 14 consecutive
       days, in situ DNA fragmentation was revealed in the neocortex but not in
       the hippocampus by terminal deoxynucleotidyl transferase (TdT)-mediated
       dUTP-biotin nick end-labelling (TUNEL). In these rats, dark degenerating
       neurones were observed in the neocortex but not in the hippocampus.
       Treatment with bovine serum albumin (300 ng/rat, i.c.v.) for up to 14
       days did not produce DNA fragmentation nor did it yield
       neuropathological lesions of the neocortex or hippocampus. In
       conclusion, the present data demonstrate that gp 120 given i.c.v.
       produced DNA fragmentation in the neocortex, thus suggesting that
       apoptosis is the mechanism through which neurones of the neocortex are
       killed.
 DE    Animal  Brain/DRUG EFFECTS/*METABOLISM  Cerebral Cortex/DRUG
       EFFECTS/*METABOLISM  Cerebral Ventricles/DRUG EFFECTS/*PHYSIOLOGY
       DNA/DRUG EFFECTS  *DNA Damage  Hippocampus/DRUG EFFECTS/METABOLISM  HIV
       Envelope Protein gp120/ADMINISTRATION & DOSAGE/*PHARMACOLOGY  *HIV-1
       Injections, Intraventricular  Male  Neurons/DRUG EFFECTS/METABOLISM
       Organ Specificity  Rats  Rats, Wistar  Recombinant
       Proteins/ADMINISTRATION & DOSAGE/PHARMACOLOGY  Support, Non-U.S. Gov't
       JOURNAL ARTICLE

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