Document 0401
 DOCN  M9590401
 TI    Codelivery to mammalian cells of a transcriptional factor with
       cis-acting element using cationic liposomes.
 DT    9509
 AU    Farhood H; Gao X; Barsoum J; Huang L; Department of Pharmacology,
       University of Pittsburgh, School of; Medicine, Pennsylvania 15261, USA.
 SO    Anal Biochem. 1995 Feb 10;225(1):89-93. Unique Identifier : AIDSLINE
       MED/95297709
 AB    The human immunodeficiency virus-1 transactivator protein (tat) was
       codelivered efficiently with a reporter gene under the control of a
       tat-responsive DNA element using different formulations of cationic
       liposomes. Expression of a tat-responsive reporter gene was induced by
       incubating cells with a mixture of purified recombinant tat protein,
       reporter DNA, and liposomes. Different cell lines were tested
       successfully as targets for the codelivery. Tat was shown to
       trans-activate the codelivered virus promoter specifically in the cells
       tested. Codelivery of tat with DNA is a useful model for studying the
       function of trans-acting factors and their cis-acting DNA elements. The
       currently available methods such as foot-printing only reveal the
       binding, but not the functional consequence of the binding, of the
       factor with the element. In addition, this system may prove useful as a
       model for high level and regulated transgene expression in target cells.
 DE    Animal  Base Sequence  Carcinoma, Squamous Cell  Cell Line
       Chloramphenicol Acetyltransferase/ANALYSIS/BIOSYNTHESIS  Comparative
       Study  CHO Cells  Drug Carriers  DNA Primers  Gene Products,
       tat/*ADMINISTRATION & DOSAGE/BIOSYNTHESIS  Hamsters  Hela Cells  Human
       HIV Long Terminal Repeat  HIV-1/*METABOLISM  Kidney  Liposomes
       Molecular Sequence Data  Mutagenesis  Phosphatidylethanolamines
       Plasmids  Polymerase Chain Reaction/METHODS  Recombinant
       Proteins/ADMINISTRATION & DOSAGE/ANALYSIS/  BIOSYNTHESIS  Sequence
       Deletion  Support, U.S. Gov't, P.H.S.  *Trans-Activation (Genetics)
       Transfection/*METHODS  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).