Document 0414
 DOCN  M9590414
 TI    Cell-to-cell fusion, but not virus entry in macrophages by T-cell line
       tropic HIV-1 strains: a V3 loop-determined restriction.
 DT    9509
 AU    Simmons G; McKnight A; Takeuchi Y; Hoshino H; Clapham PR; Virology
       Laboratory, Chester Beatty Laboratories, Institute of; Cancer Research,
       London.
 SO    Virology. 1995 Jun 1;209(2):696-700. Unique Identifier : AIDSLINE
       MED/95297176
 AB    T-cell lines chronically infected with laboratory adapted, T-cell tropic
       HIV-1 strains (RF, LAI, CBL-4, and NY5) induced syncytia after
       cocultivation with primary macrophages. Such fusion-competent strains,
       however, replicated inefficiently after cell-free infection of
       macrophage cultures. Evidence based on proviral DNA synthesis,
       pseudotype penetration, and a V3 loop mutation revealed that cell-free
       infection was restricted at a prepenetration stage in entry and
       controlled by envelope sequences. Our results suggest that primary
       macrophages are sensitive to cell-to-cell but not to virion-to-cell
       fusion induced by the envelope glycoproteins of several T-cell tropic
       HIV-1 strains.
 DE    Cell Communication  *Cell Fusion  Cell Line  Cell-Free System  Gene
       Products, env/CHEMISTRY/*METABOLISM  Giant Cells/CYTOLOGY  Human
       HIV-1/*PHYSIOLOGY  Macrophages/CYTOLOGY/*PHYSIOLOGY/VIROLOGY  Support,
       Non-U.S. Gov't  T-Lymphocytes/*PHYSIOLOGY/VIROLOGY  Virion/PHYSIOLOGY
       *Virus Replication  JOURNAL ARTICLE

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