Document 0415
 DOCN  M9590415
 TI    Mutations in the p53 tumor-suppressor gene are frequently associated
       with bovine leukemia virus-induced leukemogenesis in cattle but not in
       sheep.
 DT    9509
 AU    Dequiedt F; Kettmann R; Burny A; Willems L; Department of Molecular
       Biology and Animal Physiology, Faculty of; Agronomy, Gembloux, Belgium.
 SO    Virology. 1995 Jun 1;209(2):676-83. Unique Identifier : AIDSLINE
       MED/95297172
 AB    To investigate the mechanisms of bovine leukemia virus (BLV)-induced
       leukemogenesis, we have examined the alterations of the p53
       tumor-suppressor gene in sheep and in cattle. The sequences of the open
       reading frames as well as the intron/exon junctions of the ovine and
       bovine p53 genes were determined. Pathological samples were screened for
       the presence of p53 mutations using a single-strand conformational
       polymorphism assay. Five of ten BLV-induced bovine tumors harbored p53
       mutations. In contrast, only one of seven samples corresponding to
       circulating leukocytes from cattle in persistent lymphocytosis showed an
       alteration of the p53 gene. Surprisingly, no p53 mutation was found
       among the 10 BLV-induced sheep tumors analyzed. Altogether, these data
       indicate that p53 mutations are linked to BLV-induced leukemogenesis in
       cattle at the transition to the lymphomic stage. These results also
       enlighten different molecular mechanisms involved in sheep and in cattle
       during BLV-induced pathogenesis.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cattle  *Cattle Diseases
       Codon  Comparative Study  DNA Primers  Enzootic Bovine
       Leukosis/*GENETICS  Exons  *Genes, p53  Homozygote  Leukemia Virus,
       Bovine/*PATHOGENICITY  Molecular Sequence Data  *Point Mutation  Sheep
       *Sheep Diseases  Species Specificity  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

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