Document 0416
 DOCN  M9590416
 TI    Analysis of ALV-based packaging cell lines for production of contaminant
       defective viruses.
 DT    9509
 AU    Girod A; Cosset FL; Verdier G; Ronfort C; Centre de Genetique
       Moleculaire et Cellulaire, CNRS UMR 106,; INRA, Universite Claude
       Bernard, Villeurbanne, France.
 SO    Virology. 1995 Jun 1;209(2):671-5. Unique Identifier : AIDSLINE
       MED/95297171
 AB    We have previously described avian leukosis virus-based packaging cell
       lines that express gag, pol, and env proteins from two
       transcomplementing genomes and produce helper-free stocks of retroviral
       vectors with different host ranges. In this report, we demonstrated that
       (i) despite the deletion of the psi packaging sequence, the
       packaging-defective transcomplementing retroviral transcripts were
       packaged into virions at a level that could reach 2.3% of a wild-type
       virus packaging level and (ii) despite deletion of the 3' LTR, these
       genomes were transferred along with the vector to target cells. As these
       genomes were also bearing a selectable gene, titers of the resulting
       contaminant particles could be estimated, depending on the cell line to
       be between 0 and 6 infectious particles/ml of supernatant.
 DE    Animal  Cell Line  Defective Viruses/GENETICS/*PHYSIOLOGY  Gene
       Products, env/BIOSYNTHESIS  Gene Products, gag/BIOSYNTHESIS  Gene
       Products, pol/BIOSYNTHESIS  Genes, env  Genes, gag  Genes, pol  Genetic
       Complementation Test  Genetic Techniques  Genetic Vectors  Genome, Viral
       Support, Non-U.S. Gov't  Tissue Culture/METHODS  *Virus Replication
       JOURNAL ARTICLE

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