Document 0417
 DOCN  M9590417
 TI    Fusogenic determinants of highly cytopathic subtype D Zairian isolate
       HIV-1 NDK.
 DT    9509
 AU    De Mareuil J; Salaun D; Chermann JC; Hirsch I; Unite de Recherches sur
       les Retrovirus et Maladies Associees,; INSERM U322, Parc Scientifique et
       Technologique de Luminy,; Marseille, France.
 SO    Virology. 1995 Jun 1;209(2):649-53. Unique Identifier : AIDSLINE
       MED/95297167
 AB    Phenotypic characterization of subtype B strains of human
       immunodeficiency virus type 1 (HIV-1) indicates that the major
       determinants of their cytopathogenicity and tropism are contained in the
       gene coding for the envelope glycoprotein gp120, namely in its variable
       regions V1, V2, and V3. Recombinant viruses derived from HIV-1 LAV, the
       subtype B prototype virus, and HIV-1 NDK, the Zairian subtype D virus
       highly cytopathic for CD4-positive lymphocytes, were used to elucidate
       genetic control of fusogenic functions in subtype D viruses. Our data
       demonstrate that multigenic determination of fusogenic properties is
       more complex in the subtype D than in clade B viruses. Variability in
       three regions of HIV-1 NDK genome correlated with formation of large
       syncytia. These regions consisted of the matrix protein, the C-terminal
       portion of vpr up to the C1 region of gp120, and the V1-V3 regions of
       gp120. Variability in the envelope glycoprotein but not in other regions
       of the HIV-1 genome was related to enhanced resistance of HIV-1 NDK to
       treatment of target cells with OKT4-A anti-CD4 MAb. Therefore, a
       different genetic control affects two aspects of HIV-1 fusogenicity: (i)
       variability in the envelope glycoprotein itself is sufficient to
       influence a virus-to-cell fusion at the virus/cell entry, and (ii) a
       more complex genetic function including genes of matrix protein and
       envelope glycoprotein is related to variability of cell-to-cell fusion
       during formation of syncytium.
 DE    Antibodies, Monoclonal/PHARMACOLOGY  Comparative Study  CD4-Positive
       T-Lymphocytes/*VIROLOGY  DNA Replication  Genes, Viral  Genome, Viral
       Human  HIV Envelope Protein gp120/BIOSYNTHESIS/GENETICS  HIV-1/ISOLATION
       & PURIF/*PHYSIOLOGY/*PATHOGENICITY  Membrane Fusion  Recombination,
       Genetic  Restriction Mapping  Support, Non-U.S. Gov't  Virus Replication
       Zaire  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).