Document 0418
 DOCN  M9590418
 TI    Lack of pseudotype formation between human immunodeficiency virus type 1
       and Epstein-Barr virus in productively coinfected B lymphoblastoid cell
       lines.
 DT    9509
 AU    Van Kuyk R; Mosier DE; Department of Immunology, Scripps Research
       Institute, La Jolla,; California 92037, USA.
 SO    Virology. 1995 Jun 1;209(2):643-8. Unique Identifier : AIDSLINE
       MED/95297166
 AB    Human immunodeficiency virus (HIV) can form pseudotypes with other
       enveloped viruses, including herpes simplex virus, when the two viruses
       coinfect the same cell. Pseudotypes between HIV and Epstein-Barr virus
       (EBV) have not been described. We observed unusually high levels of
       HIV-1 replication in SCID mice transplanted with human peripheral blood
       mononuclear cells (hu-PBL-SCID mice) when the mice developed
       EBV-associated human B cell lymphoproliferative disease. If this
       enhancement of HIV-1 replication were due to pseudotype formation rather
       than direct infection of B lymphoblastic cells by HIV-1, the pseudotypes
       could pose a novel biohazard to laboratory workers. To assess whether
       HIV-1 and EBV can form such pseudotypes, we established and
       characterized CD4-positive B lymphoblastoid cell lines (LCL) that
       contained cells infected with both EBV and HIV-1. A high-titered virus
       pool from these LCL could induce HIV infection in the Burkitt's lymphoma
       (BL) line BJA-B, but not in the BL line Ramos. Infection of BJA-B was
       blocked by neutralizing antibody to HIV gp120 but not by neutralizing
       anti-EBV gp350. These experiments provide no evidence for pseudotype
       formation, suggesting a low risk for EBV:HIV pseudotypes in natural
       infection of humans or in human cells transplanted to SCID mice.
 DE    Animal  Antigens, Viral/ANALYSIS  B-Lymphocytes/*VIROLOGY  Cell Line
       CD4-Positive T-Lymphocytes/DRUG EFFECTS/IMMUNOLOGY/VIROLOGY  Herpesvirus
       4, Human/CLASSIFICATION/*PHYSIOLOGY  Human  HIV Antigens/ANALYSIS
       HIV-1/CLASSIFICATION/*PHYSIOLOGY  Lymphoproliferative
       Disorders/IMMUNOLOGY/VIROLOGY  Mice  Mice, SCID  Support, U.S. Gov't,
       P.H.S.  Tetradecanoylphorbol Acetate/PHARMACOLOGY  *Virus Replication
       JOURNAL ARTICLE

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