Document 0422
 DOCN  M9590422
 TI    Expression and characterization of proteins produced by mRNAs spliced
       into the X region of the human T-cell leukemia/lymphotropic virus type
       II.
 DT    9509
 AU    Ciminale V; D'Agostino DM; Zotti L; Franchini G; Felber BK;
       Chieco-Bianchi L; Institute of Oncology, University of Padova, Italy.
 SO    Virology. 1995 Jun 1;209(2):445-56. Unique Identifier : AIDSLINE
       GENBANK/L41677
 AB    In previous studies we showed that human T-cell leukemia/lymphotropic
       virus type I (HTLV-I) may produce novel proteins encoded in the X
       region. To investigate a possible correlation between expression of
       viral genes and different biologic properties of HTLV-I and HTLV-II, we
       analyzed expression of HTLV-II in the chronically infected cell line
       MoT. Reverse transcription-polymerase chain reaction analyses revealed
       that the virus produces several mRNAs singly or doubly spliced into the
       X region. Corresponding cDNAs were cloned and transfected into a HeLa
       cell line; resulting proteins were designated according to their sizes
       and coding open reading frames (ORFs). p10xI and p11xV were produced by
       a dicistronic doubly spliced mRNA. p10xI was generated by translation of
       the first exon of rex linked to the x-I ORF; p11xV was translated from
       the tax initiation codon linked to the x-V ORF. Two singly spliced
       polycistronic mRNAs produced p28xII, coded by the x-II ORF, and several
       isoforms generated by initiation within the x-III ORF. Studies of the
       proteins' subcellular localization revealed that they exhibited distinct
       targeting patterns. Comparison of these proteins with their HTLV-I
       counterparts indicated intriguing differences between these two viruses,
       suggesting that further study of the X region products may aid in
       defining genetic determinants of pathogenicity.
 DE    *Alternative Splicing  Amino Acid Sequence  Antigenic
       Determinants/ANALYSIS  Base Sequence  Cell Line  Comparative Study  DNA
       Primers  Genes, env  Genes, gag  Genes, pol  Genome, Viral  Human
       HTLV-I/GENETICS/METABOLISM  HTLV-II/GENETICS/*METABOLISM  Molecular
       Sequence Data  Open Reading Frames  Recombinant
       Proteins/ANALYSIS/BIOSYNTHESIS  RNA, Messenger/*METABOLISM  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Transfection  Viral
       Proteins/*BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).