Document 0423
 DOCN  M9590423
 TI    Feline immunodeficiency virus (FIV)-specific cytotoxic T lymphocytes
       from chronically infected cats are induced in vitro by retroviral
       vector-transduced feline T cells expressing the FIV capsid protein.
 DT    9509
 AU    Song W; Collisson EW; Li J; Wolf AM; Elder JH; Grant CK; Brown WC;
       Department of Veterinary Pathobiology, Texas A&M University,; College
       Station, USA.
 SO    Virology. 1995 Jun 1;209(2):390-9. Unique Identifier : AIDSLINE
       MED/95297140
 AB    We have previously reported the presence of feline immunodeficiency
       virus (FIV)-specific, major histocompatibility complex (MHC)-restricted
       cytolytic T lymphocytes (CTL) in experimentally FIV-infected cats.
       However, the fine specificity of the CTL and the role of individual FIV
       proteins in inducing FIV-specific CTL responses remain unknown. In this
       study, we examined the in vitro induction and activity of FIV p24
       capsid-specific CTL obtained from cats that had been experimentally
       infected with FIV Petaluma for 30 to 56 months. An amphotropic murine
       retroviral vector was used to generate transgenic primary feline T
       lymphoblasts that expressed the FIV capsid protein. When the autologous
       capsid-transduced T cells were used in vitro to stimulate CTL responses
       from peripheral blood mononuclear cells of chronically infected cats,
       MHC-restricted lysis of virus-infected target cells was observed. The
       majority of the CTL expressed CD8, and depletion of this population, but
       not CD4+ cells, effectively diminished the CTL activity. When the
       autologous capsid-transduced T cells were used as target cells, lysis by
       capsid-induced effectors was not observed. Analysis of capsid-transduced
       T cell clones revealed a variable and low level of capsid expression
       among the clones. This study demonstrates the potential for using
       retroviral vectors as a means of inducing CTL effector cells that will
       specifically kill lentivirus-infected cells during lentiviral infection.
 DE    Animal  Base Sequence  Capsid/*BIOSYNTHESIS  Cats  Cell Line  Cloning,
       Molecular  Cytotoxicity, Immunologic  DNA Primers  Feline Acquired
       Immunodeficiency Syndrome/*IMMUNOLOGY  Gene Expression  Immunodeficiency
       Virus, Feline/*IMMUNOLOGY/PHYSIOLOGY  Major Histocompatibility Complex
       Molecular Sequence Data  Polymerase Chain Reaction  Support, U.S. Gov't,
       P.H.S.  T-Lymphocytes, Cytotoxic/IMMUNOLOGY/*VIROLOGY  Transduction,
       Genetic  Transfection  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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