Document 0424
 DOCN  M9590424
 TI    Identification of a novel human zinc finger protein that specifically
       interacts with the activation domain of lentiviral Tat proteins.
 DT    9509
 AU    Fridell RA; Harding LS; Bogerd HP; Cullen BR; Howard Hughes Medical
       Institute, Durham, North Carolina, USA.
 SO    Virology. 1995 Jun 1;209(2):347-57. Unique Identifier : AIDSLINE
       GENBANK/U18543
 AB    Transcriptional activation of HIV-1 gene expression by the viral Tat
       protein requires the interaction of a cellular cofactor with the Tat
       activation domain. This domain has been shown to consist of the
       cysteine-rich and core motifs of HIV-1 Tat and is functionally conserved
       in the distantly related Tat proteins of HIV-2 and EIAV. Using the yeast
       two-hybrid system, we have identified a novel human gene product, termed
       HT2A, that specifically and precisely binds to the activation domain of
       HIV-1 Tat and that can also interact with the HIV-2 and EIAV Tat
       proteins in vivo. We present data further demonstrating that the
       interaction between the activation domain of HIV-1 Tat and the HT2A
       protein can be readily detected in the mammalian cell nucleus. Sequence
       analysis demonstrates that HT2A is a novel member of the C3HC4 or ring
       finger family of zinc finger proteins that includes several known
       oncogenes and transcription factors. Overall, these data suggest that
       HT2A may play a significant role in mediating the biological activity of
       the HIV-1 Tat protein in vivo.
 DE    Amino Acid Sequence  Animal  Binding Sites  Cell Line  Cell
       Nucleus/*METABOLISM  Cercopithecus aethiops  Chloramphenicol
       Acetyltransferase/ANALYSIS/BIOSYNTHESIS  Comparative Study  DNA,
       Complementary  Gene Products, tat/*METABOLISM  Hela Cells  Human
       HIV-1/GENETICS/*METABOLISM  HIV-2/*METABOLISM  Kidney  Molecular
       Sequence Data  Recombinant Proteins/ANALYSIS/BIOSYNTHESIS  Sequence
       Homology, Amino Acid  Transcription Factors/*METABOLISM  Transfection
       Zinc Fingers/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).