Document 0444
 DOCN  M9590444
 TI    Human immunodeficiency virus type 1 reverse transcriptase: enhancement
       of activity by interaction with cellular topoisomerase I.
 DT    9509
 AU    Takahashi H; Matsuda M; Kojima A; Sata T; Andoh T; Kurata T; Nagashima
       K; Hall WW; Department of Pathology, National Institute of Health,
       Tokyo,; Japan.
 SO    Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5694-8. Unique Identifier :
       AIDSLINE MED/95296378
 AB    A number of studies have suggested that topoisomerase I (topo I)
       activity may be important in human immunodeficiency virus type 1 (HIV-1)
       replication. Specifically it has been reported that purified virus
       particles have topo I activity and that inhibitors of this enzyme can
       inhibit virus replication in vitro. We have investigated a possible
       association of HIV-1 gag proteins with topo I activity. We found that
       whereas the gag-encoded proteins by themselves do not have activity, the
       nucleocapsid protein p15 can interact with and enhance the activity of
       cellular topo I. Furthermore it could be demonstrated that topo I
       markedly enhanced HIV-1 reverse transcriptase activity in vitro and that
       this could be inhibited by the topo I-specific inhibitor camptothecin.
       The findings suggest that cellular topo I plays an important role in the
       reverse transcription of HIV-1 RNA and that the recruitment of this
       enzyme may be an important step in virus replication.
 DE    Base Sequence  DNA Primers  DNA Topoisomerase/*METABOLISM  Gene
       Products, gag/PHYSIOLOGY  Hela Cells  Human
       HIV-1/*ENZYMOLOGY/GENETICS/PHYSIOLOGY  Molecular Sequence Data  Reverse
       Transcriptase/*METABOLISM  RNA, Viral/GENETICS  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Transcription, Genetic  Virus Replication
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).