Document 0446
 DOCN  M9590446
 TI    Human immunodeficiency virus 1 reservoir in CD4+ T cells is restricted
       to certain V beta subsets.
 DT    9509
 AU    Dobrescu D; Kabak S; Mehta K; Suh CH; Asch A; Cameron PU; Hodtsev AS;
       Posnett DN; Department of Medicine, Cornell University Medical College,
       New; York, NY 10021, USA.
 SO    Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5563-7. Unique Identifier :
       AIDSLINE MED/95296351
 AB    The human immunodeficiency virus 1 (HIV-1) replicates more efficiently
       in T-cell lines expressing T-cell receptors derived from certain V beta
       genes, V beta 12 in particular, suggesting the effects of a
       superantigen. The targeted V beta 12 subset was not deleted in
       HIV-1-infected patients. It was therefore possible that it might
       represent an in vivo viral reservoir. Viral load was assessed by
       quantitative PCR with gag primers and with an infectivity assay to
       measure competent virus. It was shown that the tiny V beta 12 subset
       (1-2% of T cells) often has a higher viral load than other V beta
       subsets in infected patients. Selective HIV-1 replication in V beta 12
       cells was also observed 6-8 days after in vitro infection of peripheral
       blood lymphocytes from normal, HIV-1 negative donors. Viral replication
       in targeted V beta subsets may serve to promote a biologically relevant
       viral reservoir.
 DE    Adult  Base Sequence  CD4-Positive T-Lymphocytes/*VIROLOGY  DNA Primers
       DNA, Viral/ANALYSIS  Genes, gag  Human  HIV-1/GENETICS/*ISOLATION &
       PURIF/PHYSIOLOGY/PATHOGENICITY  Molecular Sequence Data  Polymerase
       Chain Reaction  Receptors, Antigen, T-Cell, alpha-beta/*ANALYSIS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/*VIROLOGY  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).