Document 0467
 DOCN  M9590467
 TI    Isolation of HIV-1 from experimentally contaminated multidose local
       anaesthetic vials [see comments]
 DT    9509
 AU    Druce JD; Locarnini SA; Birch CJ; Victorian Infectious Diseases
       Reference Laboratory, Fairfield; Hospital, Melbourne.
 SO    Med J Aust. 1995 May 15;162(10):513-5. Unique Identifier : AIDSLINE
       MED/95295597
 CM    Comment in: Med J Aust 1995 May 15;162(10):509
 AB    OBJECTIVE: To investigate the hypothesis that HIV can be transmitted via
       contamination of multidose vials of local anaesthetic solution through
       reuse of needles and syringes. DESIGN AND SETTING: Laboratory study. (1)
       By experiments with multidose vials and disposable needles and syringes,
       we identified a sequence of events in which HIV could contaminate the
       anaesthetic solution. (2) Three anaesthetic solutions were contaminated
       with a laboratory strain of HIV and tested by viral culture and p24
       enzyme immunoassay one, two and four hours later to see how long the
       virus remained active. RESULTS: (1) Needles and syringes retained small
       volumes of fluid after use (mean, 25 microL; in syringe alone, mean 16
       microL) which could be transferred to multidose vials of local
       anaesthetic. (2) 10 mL of anaesthetic solution contaminated with 8
       microL of HIV-infected solution (equivalent to 1% infected lymphocytes
       in vivo) contained active virus one hour later. In some settings, HIV
       could be isolated four hours after exposure. CONCLUSION: When
       inadvertently contaminated with HIV, multidose solutions represent a
       potential source of transmissible virus.
 DE    Anesthetics, Local  Cell Line  Cell Transformation, Viral  Culture Media
       Drug Combinations  Drug Contamination  *Equipment Contamination  Human
       HIV Infections/TRANSMISSION/*VIROLOGY  HIV-1/*ISOLATION & PURIF  Models,
       Theoretical  Needles  Syringes  T-Lymphocytes/PATHOLOGY/*VIROLOGY  Time
       Factors  JOURNAL ARTICLE

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