Document 0481
 DOCN  M9590481
 TI    [The quantification of human immunodeficiency virus viremia in patients
       with rapid and slow progression of the disease]
 DT    9509
 AU    Bravo R; Soriano V; Martin R; del Romero J; Dronda F; Gutierrez M;
       Martinez P; Valencia E; Moreno V; Laguna F; et al; Servicio de
       Enfermedades Infecciosas, Instituto de Salud Carlos; III, Madrid.
 SO    Med Clin (Barc). 1995 Apr 15;104(14):530-4. Unique Identifier : AIDSLINE
       MED/95295385
 AB    BACKGROUND: The natural course of the human immunodeficiency virus type
       1 (HIV-1) infection is very variable. The factors which appear to
       determine the speed of immunodeficiency progression are multiple,
       although the virulence of the predominant viral strain seems to be one
       the main factors. The plasmatic viremia in individuals with rapid and
       slow HIV-1 progression was analyzed in an attempt to establish the
       degree of correlation between HIV-1 replication and the natural course
       of the disease. METHODS: Forty-two samples from 34 seropositive
       patients, 11 with rapid progression criteria (< 5 years from acute
       infection and CD4+ lymphocytes < 0.2 x 10(9)/l) and 23 with slow
       progression (> 7 years from demonstrated infection and > 0.5 x 10(9)
       CD4+ lymphocytes/l) were studied. The plasmatic viremia was quantified
       by a new method of plasma DNA genetic amplification, denominated the
       branched DNA (bDNA) technique. As a reference circulating p24 was
       determined and the presence of several proviral regions were studied in
       peripheral blood lymphocytes by polymerase chain reaction (PCR).
       RESULTS: The presence of RNA molecules was detected in plasma of 7
       (58.3%) out of 12 samples of rapid progression (RP) patients by bDNA. To
       the contrary, this was negative in 30 samples from slow progression (SP)
       patients. Four of the 5 negative RP samples corresponded to patients who
       had taken antiretroviral drugs at the time of the study. The p24
       antigenemia was positive in 5 (41.6%) from the RP patients and in none
       of the SP patients. The presence of gag, pol and env sequences was
       positive by PCR in all RP patients and in most of the SP patients.
       However, repeatedly negative results by PCR were observed in 5 SP
       samples for all or some of the genomic regions studied. CONCLUSIONS:
       Patients with rapid progression of HIV-1 have higher plasmatic viremia
       than subjects with slow disease progression.
 DE    Adult  Chi-Square Distribution  Comparative Study  Disease Progression
       DNA, Viral/GENETICS  English Abstract  Female  Gene
       Amplification/METHODS  Human  HIV Core Protein p24/BLOOD  HIV
       Infections/*DIAGNOSIS/GENETICS/IMMUNOLOGY  *HIV-1/GENETICS/IMMUNOLOGY
       Male  Polymerase Chain Reaction/STATISTICS & NUMER DATA  Prospective
       Studies  Proviruses/GENETICS  RNA, Viral/BLOOD
       Viremia/*DIAGNOSIS/GENETICS/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).