Document 0514
 DOCN  M9590514
 TI    Genetic mapping in human and mouse of the locus encoding TRBP, a protein
       that binds the TAR region of the human immunodeficiency virus (HIV-1).
 DT    9509
 AU    Kozak CA; Gatignol A; Graham K; Jeang KT; McBride OW; Laboratory of
       Molecular Microbiology, National Institutes of; Allergy and Infectious
       Diseases, National Institutes of Health,; Bethesda, Maryland 20892, USA.
 SO    Genomics. 1995 Jan 1;25(1):66-72. Unique Identifier : AIDSLINE
       MED/95293411
 AB    Productive infection with HIV-1, the virus responsible for AIDS,
       requires the involvement of host cell factors for completion of the
       replicative cycle, but the identification of these factors and
       elucidation of their specific functions has been difficult. A human
       cDNA, TRBP, was recently cloned and characterized as a positive
       regulator of gene expression that binds to the TAR region of the HIV-1
       genome. Here we demonstrate that this factor is encoded by a gene,
       TARBP2, that maps to human chromosome 12 and mouse chromosome 15, and we
       also identify and map one human pseudogene (TARBP2P) and two mouse
       TRBP-related sequences (Tarbp2-rs1, Tarbp2-rs2). The map location of the
       expressed gene identifies it as a candidate for the previously
       identified factor encoded on human chromosome 12 that has been shown to
       be important for expression of HIV-1 genes. Western blotting indicates
       that despite high sequence conservation in human and mouse, the TARBP2
       protein differs in apparent size in primate and rodent cells.
 DE    Animal  Binding Sites  *Chromosome Mapping  *Chromosomes, Human, Pair 12
       Cloning, Molecular  Cricetulus  CHO Cells  DNA, Viral/METABOLISM
       Hamsters  Hominidae/*GENETICS  Human  Hybrid Cells  *HIV Long Terminal
       Repeat  HIV-1/*METABOLISM  Mice/*GENETICS  Mice, Inbred Strains  Muridae
       Primates/GENETICS  Pseudogenes  RNA-Binding
       Proteins/*GENETICS/METABOLISM  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

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