Document 0560
 DOCN  M9590560
 TI    Selective T cell depletion with CD8-conjugated magnetic beads in the
       prevention of graft-versus-host disease after allogeneic bone marrow
       transplantation.
 DT    9509
 AU    Jansen J; Hanks S; Akard L; Martin M; Thompson J; Chang Q; Ash R;
       Garrett P; Figg F; English D; Bone Marrow Transplantation Program,
       Methodist Hospital of; Indiana, Indianapolis 46206-1367, USA.
 SO    Bone Marrow Transplant. 1995 Feb;15(2):271-8. Unique Identifier :
       AIDSLINE MED/95291229
 AB    The effects of a new immunomagnetic method of selectively depleting CD8+
       lymphocytes from donor bone marrow were studied in 29 patients
       undergoing transplantation from HLA-identical sibling (n = 20) or
       alternative (n = 9) donors. The direct immunomagnetic depletion method
       consistently removed > 95% of CD8+ cells and the non-specific loss of
       other cell subsets was only about 15%. Recovery of CFU-GM and BFU-e was
       on average > 100%. The final graft contained 0.9 +/- 0.6 x 10(8)/kg
       nucleated cells and 1.4 +/- 2.7 x 10(5)/kg CD8+ cells. Patients also
       received cyclosporine starting day -1. Engraftment occurred in 28
       patients (97%), including three patients who received a non-TBI
       conditioning regimen. One patient receiving an unrelated transplant
       failed to engraft. Median time to ANC > 500 x 10(6)/L was 17 (12-23)
       days. Four of 20 patients receiving grafts from HLA-identical siblings
       (20%) developed acute GVHD grade > or = II. However, five of eight
       patients with grafts from alternative donors (63%) had grade > or = II
       GVHD. Nearly all patients developed fever around day 7, accompanied by
       fluid overload, mild skin rash and shortness of breath. This syndrome
       necessitated treatment with steroids. Immunomagnetic CD8 depletion is a
       simple and reproducible method of selective T cell depletion. In
       combination with cyclosporine it appears to be effective in the
       prevention of severe acute GVHD in HLA-identical sibling transplants,
       but not in transplants from less perfectly matched donors.
 DE    Adult  Antigens, CD8/*THERAPEUTIC USE  Bone Marrow
       Transplantation/*ADVERSE EFFECTS/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY/*PATHOLOGY  Female  Graft vs Host
       Disease/IMMUNOLOGY/*PREVENTION & CONTROL  Human  Immunomagnetic
       Separation  Male  Middle Age  T-Lymphocyte Subsets/PATHOLOGY
       Transplantation, Homologous/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).