Document 0567
 DOCN  M9590567
 TI    Cryptic dysfunction of cellular immunity in asymptomatic human
       immunodeficiency virus (HIV) carriers and its actualization by an
       environmental immunosuppressive factor.
 DT    9509
 AU    Sugiura M; Imai S; Sakurada K; Miyazaki T; Osato T; Department of
       Virology, Hokkaido University School of Medicine,; Sapporo, Japan.
 SO    In Vivo. 1994 Nov-Dec;8(6):1019-22. Unique Identifier : AIDSLINE
       MED/95290636
 AB    Peripheral blood lymphocytes from 4 asymptomatic HIV-1 carriers with
       normally retained EBV-specific cytotoxic T-cell activity were exposed to
       EBV and incubated with 0.2 ng/ml 4-deoxyphorbol ester, an
       immunosuppressive substance derived from an African plant Euphorbia
       tirucalli. The regression of EBV-induced B-cell transformation by
       EBV-specific cytotoxic T-cells was significantly impaired in the
       presence of a small amount of 4-deoxyphorbol ester, but not so in 5
       HIV-1-seronegative healthy counterparts. When the EBV-specific cytotoxic
       T-cells from the asymptomatic carriers were exposed to 0.2ng/ml
       4-deoxyphorbol ester and incubated with 51Cr-labeled autologous
       EBV-transformed B lymphocytes, the released radioactivity was
       significantly smaller than that of the healthy counterparts. The results
       suggest that the cellular immunity of the asymptomatic HIV-1 carriers is
       cryptically impaired, and the cryptic immunological dysfunction is
       actualized by exposure to a small amount of the immunosuppressive
       substance, a dose which does not affect the immunity of uninfected
       healthy individuals.
 DE    Adolescence  Adult  Carrier State  Cell Line  Female  Herpesvirus 4,
       Human/*IMMUNOLOGY  Human  HIV Seropositivity/*IMMUNOLOGY  *HIV-1
       Immunity, Cellular/*DRUG EFFECTS  Male  Middle Age  Phorbol
       Esters/PHARMACOLOGY  Support, Non-U.S. Gov't  T-Lymphocytes,
       Cytotoxic/CYTOLOGY/*DRUG EFFECTS/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).