Document 0692
 DOCN  M9590692
 TI    Integration of incomplete hiv-1 reverse transcription products.
 DT    9509
 AU    Miller MD; Wang B; Bushman FD; Infectious Disease Laboratory, The Salk
       Institute for Biological; Studies, La Jolla, CA
 SO    NIH Conf Retroviral Integrase. 1995 Jan 19-20;:(Participants' abstracts
       and posters, abstract no. 6a). Unique Identifier : AIDSLINE
       AIDS/95920027
 AB    Despite intensive study, the mechanism by which many retroviruses
       complete reverse transcription has remained unclear. Many retroviruses
       and all lentiviruses fail to complete the synthesis of the second strand
       of the viral cDNA (plus strand) efficiently in infected cells. For HIV-
       1, we find in single step infection experiments that complete plus
       strands are rare (<1% of products) at times when integration is taking
       place. Subviral nucleoprotein complexes containing such incomplete cDNA
       can be extracted from infected cells and used to generate integration
       products in vitro. Analysis of such integration products using
       two-dimensional gel electrophoresis revealed that the discontinuous
       viral DNA was efficiently integrated into an added target DNA. These
       data support a model in which discontinuities in the plus strand need
       not be sealed until after integration, potentially by the host cell
       enzymes that are already thought to repair DNA gaps at the junctions
       between host and viral DNA.
 DE    DNA Nucleotidyltransferases/*METABOLISM  DNA,
       Single-Stranded/*METABOLISM  DNA-Binding Proteins/METABOLISM
       HIV-1/*ENZYMOLOGY  Oligonucleotides/*METABOLISM  Repetitive Sequences,
       Nucleic Acid  Substrate Specificity  MEETING ABSTRACT

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