Document 0714
 DOCN  M9590714
 TI    NIH Conference Retroviral Integrase.
 DT    9509
 AU    Skalka AM
 SO    NIH Conf Retroviral Integrase. 1995 Jan 19-20;:(Session II, speakers'
       abstracts - unpaged). Unique Identifier : AIDSLINE AIDS/95920005
 AB    The general outline of the integration pathway has been known for some
       time. DNA is synthesized within a subviral particle that is deposited in
       the cytoplasm of the infected cell after entry and uncoating of the
       virion. The first biochemical step, an endonucleolytic processing by
       which IN removes two nucleotides from the 3' ends of this DNA, can take
       place immediately after DNA synthesis is completed. The resulting
       preintegration complex must then make its way to the nucleus of the
       infected cell where the second biochemical step takes place: a concerted
       cleavage and ligation that results in the joining of viral and cell DNA.
       The development of reconstituted in vitro systems that employ purified
       IN and model DNA substrates have made it possible to explore several
       aspects of the biochemistry of this reaction. However, many questions
       remain concerning the organization and structure of components in vivo,
       such as how viral and cell components interact, and how the structure of
       IN relates to the various functions of the enzyme. This report will
       present an update on our most recent findings concerning the structure
       of IN and its relationship to activities measured both in vitro and in
       vivo. They include the identification of a nuclear localization signal
       and self-association determinants in RSV IN, and the construction and
       analysis of novel substrates that may provide clues to the organization
       of viral DNA ends in the coordinated processing reaction that occurs in
       vivo.
 DE    Cytoplasm/VIROLOGY  DNA Nucleotidyltransferases/*METABOLISM  DNA,
       Viral/*GENETICS/METABOLISM  Protein Processing, Post-Translational
       Retroviridae/*ENZYMOLOGY  Virion  Virus Integration/*GENETICS  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).