Document 0715
 DOCN  M9590715
 TI    Genetic footprinting: using a retroviral integrase to study gene
       function.
 DT    9509
 AU    Brown PO; Singh I; Crowley R; Department of Biochemistry, Stanford
       University; HHMI
 SO    NIH Conf Retroviral Integrase. 1995 Jan 19-20;:(Session I, speakers'
       abstracts - unpaged). Unique Identifier : AIDSLINE AIDS/95920004
 AB    As an approach to characterising the early steps in retroviral infection
       and discovering the roles viral proteins play in those steps, we would
       like to screen a large set of mutations in the viral genome for specific
       defects in this phase of the life cycle. Because the mutants of interest
       are expected to be rare, and cannot be positively selected or recovered
       as infectious virions, this kind of screen presents a particularly
       challenging version of the general problem of studying a gene's function
       by identifying mutants with specific defects. To make such an ambitious
       screen more practical and more powerful, we have been developing a new
       experimental approach to making and analyzing a large set of mutations
       in a cloned DNA sequence that, ironically, uses a retroviral integrase
       as a tool. Preliminary results, using a model gene, will be presented,
       and potential applications to the study of retroviral replication will
       be discussed.
 DE    Cloning, Molecular  DNA Nucleotidyltransferases/*METABOLISM  DNA,
       Viral/*GENETICS/METABOLISM  Genome, Viral  Mutation
       Retroviridae/*ENZYMOLOGY  Retroviridae
       Infections/GENETICS/*PHYSIOPATHOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).