Document 0717
 DOCN  M9590717
 TI    A human homologue of the yeast snf5 transcription factor binds and
       stimulates the hiv-1 integrase
 DT    9509
 AU    Goff SP; Kalpana GV; Howard Hughes Medical Institute, Dept of
       Biochemistry and; Molecular Biophysics, Columbia University College of P
       & S, New; York, NY
 SO    NIH Conf Retroviral Integrase. 1995 Jan 19-20;:(Session I, speakers'
       abstracts - unpaged). Unique Identifier : AIDSLINE AIDS/95920002
 AB    We have used the yeast two-hybrid system to identify a novel human gene
       product that binds tightly to the HIV-1 integrase in vitro. The sequence
       of the gene suggests that the novel protein is a human homologue of
       yeast SNF5, a transcriptional activator required for high level
       expression of many genes. The mammalian protein is present as part of a
       large complex, the SNF/SWI complex, which relieves chromatin-induced
       repression of transcription templates. The 44-kD protein is very widely
       expressed in various tissue and cell lines. Recombinant forms of the
       protein, and the native complex isolated from mammalian nuclei, both
       stimulate the DNA joining activity of the HIV-1 IN. We propose that the
       new gene, termed INI1 for integrase interactor 1, may encode a nuclear
       host factor that activates the viral integration complex. The protein
       may promote integration and/or target incoming viral DNA to active
       genes.
 DE    DNA Nucleotidyltransferases/*METABOLISM  DNA, Viral/GENETICS/METABOLISM
       Enzyme Activation  HIV-1/*ENZYMOLOGY  Human  Nuclear Proteins/GENETICS
       Protein Binding  Recombinant Proteins/GENETICS/METABOLISM  Saccharomyces
       cerevisiae/*GENETICS  Templates  Transcription
       Factors/*GENETICS/METABOLISM  Virus Integration/GENETICS  MEETING
       ABSTRACT

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