Document 0730
 DOCN  M9590730
 TI    Immunohistochemical analysis of T cell phenotypes in patients with
       graft-versus-host disease following allogeneic bone marrow
       transplantation.
 DT    9509
 AU    Diamond DJ; Chang KL; Jenkins KA; Forman SJ; Department of Hematology
       and Bone Marrow Transplantation, City of; Hope National Medical Center,
       California, USA.
 SO    Transplantation. 1995 May 27;59(10):1436-44. Unique Identifier :
       AIDSLINE MED/95288801
 AB    Allogeneic bone marrow transplantation has become the therapy of choice
       in many cases of hematologic malignancy. In both matched related donor
       transplants--and, to a greater degree, in unrelated donor transplant
       situations--a major complication of the procedure is GVHD. This problem
       is caused by mature T cells in the graft, which also facilitate
       engraftment, and mediate an antitumor effect to reduce relapse. In order
       to further characterize the T cells that are present at the GVHD site of
       injury, we have studied 134 fresh tissue biopsies using
       immunohistochemical methods from 50 consecutive ABMT recipients
       clinically suspected of having acute GVHD. Antibodies specific for T
       cells, T cell receptor subsets, B cells, and NK cells were used to
       characterize the lymphocytic infiltrate in the biopsy tissue from GVHD
       patients. The data showed that the majority of lymphocytes that had
       infiltrated the epithelium or epidermis were CD3+ T cells. Using
       antibodies that distinguished the alpha/beta (beta F1) from the
       gamma/delta TCR (TCR delta 1)-expressing T cells, we observed that the
       lymphocytic infiltrates from involved tissues of the gastrointestinal
       tract, skin, and liver are almost exclusively derived from the
       alpha/beta expressing T cell subset, and are of the memory cell subset
       of T cells (CD45RO). This is in contrast to some examples from other
       disease states, in which a significant proportion of the lymphocytes
       that infiltrate the epidermal layers are of the gamma/delta type.
 DE    Adolescence  Adult  Biopsy  Bone Marrow Transplantation/*ADVERSE EFFECTS
       CD4-Positive T-Lymphocytes/CYTOLOGY  CD8-Positive T-Lymphocytes/CYTOLOGY
       Gastrointestinal System/CHEMISTRY/PATHOLOGY/ULTRASTRUCTURE  Graft vs
       Host Disease/*ETIOLOGY/*GENETICS  Human  *Immunohistochemistry
       Immunologic Memory/IMMUNOLOGY  Liver/PATHOLOGY  Middle Age  Phenotype
       Receptors, Antigen, T-Cell, alpha-beta/ANALYSIS
       Skin/CHEMISTRY/PATHOLOGY/ULTRASTRUCTURE  Support, U.S. Gov't, P.H.S.
       T-Lymphocyte Subsets/CYTOLOGY  T-Lymphocytes/CHEMISTRY/*METABOLISM
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).