Document 0739
 DOCN  M9590739
 TI    Intestinal disaccharidase activity in human immunodeficiency virus
       disease.
 DT    9509
 AU    Lim SG; Menzies IS; Nukajam WS; Lee CA; Johnson MA; Pounder RE;
       University Dept. of Medicine, Haemophilia Centre, London, UK.
 SO    Scand J Gastroenterol. 1995 Mar;30(3):235-41. Unique Identifier :
       AIDSLINE MED/95288569
 AB    BACKGROUND: The relationship between disaccharidase activity,
       progression of human immunodeficiency virus (HIV) disease, and diarrhoea
       and weight loss was investigated. METHODS: Forty-six HIV-positive
       patients ingested a solution of lactose, palatinose, sucrose, and
       lactulose after 24 h of dietary exclusion and overnight fasting, after
       which urine was collected for 10 h. Urinary disaccharide (activity)
       ratios-lactose/lactulose (L/LL), palatinose/lactulose (P/LL), and
       sucrose/lactulose (S/LL)-were measured by thin-layer chromatography.
       RESULTS: There was a significant decrease in disaccharidase activity
       (L/LL, P/LL, and S/LL) with advancing clinical stage of HIV disease (p <
       0.05, Wilcoxon rank sum test) as well as decreasing CD4 count (p < 0.05,
       Spearman correlation). Patients with weight loss/diarrhoea also had
       significantly (p < 0.05) decreased disaccharidase activity compared with
       control but not as compared with AIDS patients. Anti-retroviral therapy
       did not influence disaccharidase activity. CONCLUSION: Impairment of
       disaccharidase activity occurs with advancing HIV disease, but its role
       in HIV patients with weight loss and diarrhoea remains to be determined.
 DE    Adult  Case-Control Studies  Chromatography, Thin Layer  Comparative
       Study  CD4 Lymphocyte Count  Diarrhea/ETIOLOGY
       Disaccharidases/*METABOLISM  Disaccharides/DIAGNOSTIC USE/URINE  Human
       HIV Infections/COMPLICATIONS/*ENZYMOLOGY/IMMUNOLOGY  Intestine,
       Small/*ENZYMOLOGY  Male  Weight Loss  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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