Document 0774
 DOCN  M9590774
 TI    Use of a novel human immunodeficiency virus type 1 reporter virus
       expressing human placental alkaline phosphatase to detect an alternative
       viral receptor.
 DT    9509
 AU    He J; Landau NR; Aaron Diamond AIDS Research Center, New York University
       School of; Medicine, New York 10016, USA.
 SO    J Virol. 1995 Jul;69(7):4587-92. Unique Identifier : AIDSLINE
       MED/95287526
 AB    We report here on the construction and use of a novel human
       immunodeficiency virus (HIV) type 1 reporter vector, HIV-AP, that
       encodes human placental alkaline phosphatase. Upon staining with
       chromogenic alkaline phosphatase substrates 24 to 36 h postinfection,
       cells infected with HIV-AP develop an intense purple color and can then
       be counted under a dissecting microscope. Alternatively, HIV-AP
       infectivity can be quantitated and infected cells can be sorted by a
       fluorescence-activated cell sorter after staining with a fluorescent
       alkaline phosphatase substrate. The assay is rapid and accurate, has
       very low background in a variety of cell lines and primary cells, and is
       not restricted to use in human cells. Infectious HIV-AP can be
       pseudotyped by various HIV or murine leukemia virus envelope
       glycoproteins. Using this virus, we have addressed the long-standing
       question of CD4-independent infection of cells by HIV. Our results
       confirm the presence on a human osteosarcoma cell line of an alternative
       receptor for HIV infection that functions with an efficiency
       approximately 1/20 that of CD4.
 DE    Alkaline Phosphatase/*GENETICS  Antibodies, Monoclonal/IMMUNOLOGY
       Antigens, CD4/PHYSIOLOGY  Base Sequence  Flow Cytometry  Gene Products,
       env/PHYSIOLOGY  Genes, Reporter  Human  HIV-1/*PHYSIOLOGY
       Isoenzymes/*GENETICS  Molecular Sequence Data  Receptors, HIV/*ANALYSIS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).