Document 0777
 DOCN  M9590777
 TI    Monocyte-derived cultured dendritic cells are susceptible to human
       immunodeficiency virus infection and transmit virus to resting T cells
       in the process of nominal antigen presentation.
 DT    9509
 AU    Tsunetsugu-Yokota Y; Akagawa K; Kimoto H; Suzuki K; Iwasaki M; Yasuda S;
       Hausser G; Hultgren C; Meyerhans A; Takemori T; Department of
       Immunology, National Institute of Health and AIDS; Research Center,
       Tokyo, Japan.
 SO    J Virol. 1995 Jul;69(7):4544-7. Unique Identifier : AIDSLINE
       MED/95287517
 AB    The susceptibility of monocyte-derived cultured dendritic cells (DCs) to
       human immunodeficiency virus (HIV) infection and their role in viral
       transmission in the immune response were studied in detail. We observed
       that highly purified cultured DCs were infected with the T-tropic Lai
       strain of HIV type 1 (HIV-1Lai) via the CD4 receptor, and this was
       followed by formation of the complete provirus as detected by PCR. HIV
       mRNAs were transcribed at only low levels, and virus production was
       undectable; however, the addition of the purified protein derivative
       antigen of tuberculin and of autologous resting T cells to
       HIV-1Lai-infected DCs but not to HIV-1Lai-infected macrophages led to
       massive HIV transmission and production. These data suggest that the
       interaction of infected DCs with T cells during the normal immune
       response could play an important role in the activation and expansion of
       HIV.
 DE    *Antigen Presentation  Base Sequence  Cell Communication  Cells,
       Cultured  Dendritic Cells/*VIROLOGY  Human  HIV Core Protein
       p24/BIOSYNTHESIS  HIV-1/*PHYSIOLOGY  Molecular Sequence Data
       Monocytes/*VIROLOGY  RNA, Messenger/ANALYSIS  Support, Non-U.S. Gov't
       T-Lymphocytes/*VIROLOGY  JOURNAL ARTICLE

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