Document 0779
 DOCN  M9590779
 TI    Isolation and characterization of a syncytium-inducing,
       macrophage/T-cell line-tropic human immunodeficiency virus type 1
       isolate that readily infects chimpanzee cells in vitro and in vivo.
 DT    9509
 AU    Shibata R; Hoggan MD; Broscius C; Englund G; Theodore TS; Buckler-White
       A; Arthur LO; Israel Z; Schultz A; Lane HC; et al; Laboratory of
       Molecular Microbiology, National Institute of; Allergy and Infectious
       Diseases, Bethesda, MD 20892-0460, USA.
 SO    J Virol. 1995 Jul;69(7):4453-62. Unique Identifier : AIDSLINE
       MED/95287502
 AB    Fresh human immunodeficiency virus type 1 (HIV-1) isolates from patients
       with AIDS were screened for infectivity in chimpanzee peripheral blood
       mononuclear cells (PBMC) to identify strains potentially able to
       generate high virus loads in an inoculated animal. Only 3 of 23 isolates
       obtained were infectious in chimpanzee cells. Of these three, only one
       (HIV-1DH12) was able to initiate a productive infection in PBMC samples
       from all 25 chimpanzees tested. HIV-1DH12 tissue culture infections were
       characterized by extremely rapid replication kinetics, profound
       cytopathicity, and tropism for chimp and human PBMC, primary human
       macrophage, and several human T-cell lines. An infection was established
       within 1 week of inoculating a chimpanzee with 50 50% tissue culture
       infective doses of HIV-1DH12; cell-free virus was recovered from the
       plasma at weeks 1, 2, and 4 and was associated with the development of
       lymphadenopathy. Virus loads during the primary infection and at 6
       months postinoculation were comparable to those reported in
       HIV-1-seropositive individuals.
 DE    Acquired Immunodeficiency Syndrome/VIROLOGY  Adult  Amino Acid Sequence
       Animal  Base Sequence  Cell Line  Chimpansee troglodytes  Cloning,
       Molecular  DNA, Viral/GENETICS  Human  HIV-1/ISOLATION &
       PURIF/*PATHOGENICITY  Macrophages/*VIROLOGY  Male  Molecular Sequence
       Data  T-Lymphocytes/*VIROLOGY  JOURNAL ARTICLE

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