Document 0783
 DOCN  M9590783
 TI    Detection of CD4+ T cells harboring human immunodeficiency virus type 1
       DNA by flow cytometry using simultaneous immunophenotyping and
       PCR-driven in situ hybridization: evidence of epitope masking of the CD4
       cell surface molecule in vivo.
 DT    9509
 AU    Patterson BK; Goolsby C; Hodara V; Lohman KL; Wolinsky SM; Department of
       Medicine, Northwestern University Medical School,; Chicago, Illinois
       60611, USA.
 SO    J Virol. 1995 Jul;69(7):4316-22. Unique Identifier : AIDSLINE
       MED/95287486
 AB    Human immunodeficiency virus type 1 (HIV-1) infection of T cells and
       cells of the monocyte/macrophage lineage requires a specific interaction
       between the CD4 antigen expressed on the cell surface and the HIV-1
       external envelope glycoprotein (gp120). To study the association between
       HIV-1 infection and modulation of cell surface expression of the CD4
       molecule in vivo, we examined the CD4+ T cells harboring proviral DNA
       obtained from HIV-1-infected individuals who had received no
       antiretroviral therapy for at least 90 days. Simultaneous
       immunophenotyping of CD4 cell surface expression and PCR-driven in situ
       hybridization for HIV-1 DNA were used to resolve the CD4+ T cells into
       distinct populations predicted upon the presence or absence of proviral
       DNA. Among the HIV-1-infected study subjects, the percentage of CD4+ T
       cells harboring proviral DNA ranged from 17.3 to 55.5%, with a mean of
       40.5%. Cell surface fluorescent staining with anti-CD4 antibody directed
       against a non-gp120 binding site-related epitope (L120) or a
       conformation-dependent epitope of the gp120 binding site (Leu 3A)
       demonstrated either an equivalent or a 1.5- to 3-fold-lower cell surface
       staining intensity for the HIV-1 DNA-positive subpopulation relative to
       the HIV-1 DNA-negative subpopulation, respectively. These data suggest
       that masking or alteration of specific epitopes on the CD4 molecule
       occurs after viral infection.
 DE    *Antigenic Determinants  Antigens, CD4/*ANALYSIS  Cell Line
       CD4-Positive T-Lymphocytes/*VIROLOGY  DNA, Viral/*ANALYSIS  Flow
       Cytometry  Human  HIV-1/GENETICS/*ISOLATION & PURIF  Immunophenotyping
       In Situ Hybridization  Polymerase Chain Reaction  Proviruses/GENETICS
       Receptors, Antigen, T-Cell/PHYSIOLOGY  Signal Transduction  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).