Document 0795
 DOCN  M9590795
 TI    Incorporation of pseudorabies virus gD into human immunodeficiency virus
       type 1 Gag particles produced in baculovirus-infected cells.
 DT    9509
 AU    Garnier L; Ravallec M; Blanchard P; Chaabihi H; Bossy JP; Devauchelle G;
       Jestin A; Cerutti M; Laboratorie de Pathologie Comparee, Centre National
       de la; Recherche Scientifique UA 1184, France.
 SO    J Virol. 1995 Jul;69(7):4060-8. Unique Identifier : AIDSLINE
       MED/95287455
 AB    The human immunodeficiency virus type 1 (HIV-1) Pr55gag precursors were
       previously shown to assemble and bud efficiently as noninfectious
       virus-like particles (VLPs) when expressed in baculovirus-infected
       insect cells. In this study, we examined the abilities of foreign
       antigens to be incorporated on the outer surface of HIV-1 Gag particles.
       We have used a dual recombinant baculovirus, expressing the HIV-1 Gag
       gene and gD gene under the control of the P10 and polyhedrin promoters,
       respectively, to obtain hybrid VLPs. Transmission electron microscopy of
       insect cells infected with the dual recombinant revealed very large
       aggregates of particles budding from the cell membrane. The release of
       VLPs into the culture medium was clearly different for a recombinant
       baculovirus producing solely HIV-1 Gag, for which particles were
       uniformly distributed all around the cell surface. Biochemical analysis
       of hybrid particles indicated that glycoprotein gD was packaged into
       HIV-1 Gag VLPs. Moreover, the carboxy-terminal p6 region of Gag
       polyprotein and the glycoprotein gD intracytoplasmic domain were not
       required for gD incorporation. The experiments described here clearly
       demonstrate that glycoprotein gD can be packaged with HIV-1 Gag
       particles and released from insect cells.
 DE    Animal  Baculoviridae/GENETICS  Cells, Cultured  Gene Products,
       gag/*METABOLISM  HIV-1/*METABOLISM  Recombinant Proteins/METABOLISM
       Spodoptera  Viral Envelope Proteins/*METABOLISM  JOURNAL ARTICLE

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