Document 0806
 DOCN  M9590806
 TI    Induction of human immunodeficiency virus type 1 (HIV-1)-specific
       cytolytic T lymphocyte responses in seronegative adults by a
       nonreplicating, host-range-restricted canarypox vector (ALVAC) carrying
       the HIV-1MN env gene.
 DT    9509
 AU    Egan MA; Pavlat WA; Tartaglia J; Paoletti E; Weinhold KJ; Clements ML;
       Siliciano RF; Department of Medicine, School of Medicine, Johns Hopkins;
       University, Baltimore, Maryland 21205, USA.
 SO    J Infect Dis. 1995 Jun;171(6):1623-7. Unique Identifier : AIDSLINE
       MED/95287062
 AB    CD8+ cytolytic T lymphocytes (CTL) are likely to be an important
       component of effective vaccines against human immunodeficiency virus
       type 1 (HIV-1). CTL can be induced most effectively with live virus
       vectors. However, because of concerns about the safety of such vectors,
       a nonreplicating canarypox vector (ALVAC) capable of expressing foreign
       genes in mammalian cells has been developed. This study evaluated the
       capacity of an ALVAC vector expressing the HIV-1MN envelope (env)
       glycoprotein to induce HIV-1-specific CTL in seronegative volunteers.
       Protocols were designed to determine whether immunization with ALVAC
       alone or in combination with subunit boosting could induce CTL in
       vaccinia-immune and -naive volunteers. A simple method for
       antigen-specific in vitro stimulation was used to detect CTL responses
       in HIV-1-seronegative vaccine recipients. The results indicate that low
       doses of a nonreplicating virus vector alone can elicit both CD4+ and
       CD8+ HIV-1-specific CTL in a subset of seronegative volunteers.
 DE    Adult  Avipoxvirus/*GENETICS  AIDS Vaccines/*IMMUNOLOGY  Cytotoxicity,
       Immunologic  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  *Genes, env  Genetic Vectors  Human
       HIV-1/*IMMUNOLOGY  Immunity, Cellular  Support, U.S. Gov't, P.H.S.
       T-Lymphocyte Subsets/IMMUNOLOGY  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       Time Factors  Vaccines, Synthetic  JOURNAL ARTICLE

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