Document 0807
 DOCN  M9590807
 TI    Single-strand conformation polymorphism study of human immunodeficiency
       virus type 1 RNA and DNA in plasma, peripheral blood mononuclear cells,
       and their virologic cultures.
 DT    9509
 AU    Lin HJ; Siwak EB; Lauder IJ; Hollinger FB; Division of Molecular
       Virology, Baylor College of Medicine,; Houston, TX 77030, USA.
 SO    J Infect Dis. 1995 Jun;171(6):1619-22. Unique Identifier : AIDSLINE
       MED/95287061
 AB    Single-strand conformation polymorphism (SSCP) analysis was applied to
       human immunodeficiency virus type 1 (HIV-1) nucleic acids in plasma and
       peripheral blood mononuclear cells (PBMC) from 16 patients and to 15
       PBMC cocultures and 6 plasma cultures prepared from the specimens. Two
       hypervariable regions were analyzed: in the gag gene and part of the V3
       loop. Random paired matching of SSCP patterns between HIV-1 RNA and
       provirus DNA was tested, from plasma and PBMC from the same blood
       specimen, supernatant and PBMC from the same PBMC coculture, supernatant
       and PBMC from the same plasma culture, provirus DNA in cocultured PBMC
       and the PBMC inoculum, and HIV-1 RNA in a plasma culture supernatant and
       in the plasma inoculum. Paired matching was nonrandom for both regions
       in the first three situations and for gag in the fourth, with P < or =
       .01; matching was random for gag in the last situation. The HIV-1 env
       target region produced in culture diverged from that in the inoculum in
       18 of 21 instances.
 DE    Base Sequence  DNA Primers/CHEMISTRY  DNA, Viral/*GENETICS  Genes, env
       Genes, gag  Human  HIV Seropositivity/*MICROBIOLOGY  HIV-1/*GENETICS
       Leukocytes, Mononuclear/MICROBIOLOGY  Molecular Sequence Data
       *Polymorphism, Single-Stranded Conformational  RNA, Viral/*GENETICS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).