Document 0810
 DOCN  M9590810
 TI    Ganciclovir therapy for cytomegalovirus (CMV) infection of the central
       nervous system in AIDS patients: monitoring by CMV DNA detection in
       cerebrospinal fluid.
 DT    9509
 AU    Cinque P; Baldanti F; Vago L; Terreni MR; Lillo F; Furione M; Castagna
       A; Monforte AD; Lazzarin A; Linde A; Department of Infectious Diseases,
       IRCCS Ospedale San Raffaele,; Milan, Italy.
 SO    J Infect Dis. 1995 Jun;171(6):1603-6. Unique Identifier : AIDSLINE
       MED/95287057
 AB    Seven AIDS patients with central nervous system (CNS) disease and
       cytomegalovirus (CMV) DNA in cerebrospinal fluid (CSF) were evaluated
       before and 3 weeks after standard ganciclovir treatment by nested
       polymerase chain reaction (PCR) on limiting dilutions and by
       quantitative PCR. After therapy, PCR CSF was negative for CMV in 3
       patients with low baseline levels of CMV DNA and positive with decreased
       DNA titers in 4 patients with higher baseline levels. CMV pp65 antigen
       in polymorphonuclear leukocytes was found in 6 of 7 patients before
       therapy and in none of 5 after therapy. At autopsy, CMV was found in the
       CNS of the 4 cases examined, including 3 whose CSF was continuously
       positive by PCR. Quantitative PCR of CSF is useful for monitoring
       ganciclovir treatment in CMV infection of the CNS. In AIDS patients,
       standard ganciclovir treatment seems effective in reducing but not
       suppressing viral replication in severe cases of CMV infection of the
       CNS.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS/DRUG THERAPY  Central
       Nervous System Diseases/*DRUG THERAPY  Cytomegalovirus
       Infections/DIAGNOSIS/*DRUG THERAPY  Cytomegalovirus Retinitis/DRUG
       THERAPY  DNA, Viral/CEREBROSPINAL FLUID  Ganciclovir/*THERAPEUTIC USE
       Human  Polymerase Chain Reaction  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

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