Document 0812
 DOCN  M9590812
 TI    Dependency of antibody titer on provirus load in human T lymphotropic
       virus type I carriers: an interpretation for the minor population of
       seronegative carriers.
 DT    9509
 AU    Miyata H; Kamahora T; Iha S; Katamine S; Miyamoto T; Hino S; Department
       of Virology, Faculty of Medicine, Tottori University,; Yonago, Japan.
 SO    J Infect Dis. 1995 Jun;171(6):1455-60. Unique Identifier : AIDSLINE
       MED/95287035
 AB    To evaluate the prevalence of seronegative carriers of human T
       lymphotropic virus type I (HTLV-I), buffy coat samples from 1015
       Okinawan high school students were tested by immunoassays and nested
       polymerase chain reaction (PCR). Among 17 HTLV-I carriers, 1 person who
       was seronegative and 1 who was PCR-negative were identified. gag and
       tax/rex PCR titers correlated with each other (r = .92; P < .001). Of
       the 17 carriers, 14 (82%) had high virus loads (geometric averages, 522
       gag and 703 tax/rex copies/micrograms of DNA; 95% confidence intervals,
       38-7260 and 75-6594, respectively). Carriers with low virus loads had <
       or = 2.2 gag copies. In the high-virus-load group, the gag PCR titers
       correlated with the antibody titers (r = 0.88; P < .001). The regression
       line intersected the minimum antibody detection level at 35 gag
       copies/micrograms of DNA. These results suggest that a small percentage
       of carriers may be seronegative.
 DE    Adolescence  Adult  Base Sequence  Carrier State  DNA Primers/CHEMISTRY
       DNA, Viral/ANALYSIS  Female  Human  HTLV-BLV Antibodies/*IMMUNOLOGY
       HTLV-I/GENETICS/*IMMUNOLOGY  HTLV-I Infections/*IMMUNOLOGY/MICROBIOLOGY
       Japan  Male  Maternal-Fetal Exchange  Molecular Sequence Data
       Polymerase Chain Reaction/METHODS  Pregnancy  Proviruses/GENETICS
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).