Document 0820
 DOCN  M9590820
 TI    Retrovirus-like activity in an immunosuppressed dog: pathological and
       immunological findings.
 DT    9509
 AU    Modiano JF; Getzy DM; Akol KG; Van Winkle TJ; Cockerell GL; Department
       of Pediatrics, National Jewish Center for Immunology; and Respiratory
       Medicine, Denver, CO 80206, USA.
 SO    J Comp Pathol. 1995 Feb;112(2):165-83. Unique Identifier : AIDSLINE
       MED/95286875
 AB    A putative retrovirus was isolated from a dog with a severe, acquired
       immunodeficiency-like syndrome. The haematological abnormalities and
       immunological deficiencies included anaemia, leucopenia (lymphopenia and
       neutropenia), thrombocytopenia, decreased humoral immunity, and
       ineffective T-cell responses in-vitro. The necropsy findings included
       generalized lymphoid depletion, severe bone marrow hypoplasia,
       plasmacytic infiltrates in lymphoid and non-lymphoid organs, and severe
       secondary infections. Supernates of peripheral blood mononuclear cell
       cultures from the affected dog contained an agent with
       manganese-dependent reverse transcriptase (RT) activity that sedimented
       at a density of 1.122 g/ml. RT activity was also found post-mortem in
       extracts prepared from the bone marrow, lymph nodes, and small
       intestine. The lymph nodes and small intestine expressed a 3.8 kb mRNA
       that was recognized by a bovine leukaemia virus (BLV) pol DNA probe by
       Northern blotting. DNA isolated from the lymph nodes and small intestine
       from the affected dog showed distinct band patterns by Southern
       analysis, suggesting an exogenous retrovirus. The retrovirus could be
       propagated in normal canine peripheral blood mononuclear cells or
       short-term canine lymphocyte cell lines in-vitro, and was cytopathogenic
       for cells of canine, but not human, origin. These results suggest the
       existence of a pathogenic canine retrovirus capable of producing disease
       of the type associated with retroviruses in other species.
 DE    Animal  Bone Marrow/ENZYMOLOGY/PATHOLOGY  Case Report  Cell Line  Cells,
       Cultured  Dog Diseases/IMMUNOLOGY/PATHOLOGY/*VIROLOGY  Dogs  DNA,
       Viral/ANALYSIS  Female  Human  Immunologic Deficiency
       Syndromes/IMMUNOLOGY/PATHOLOGY/*VETERINARY/  VIROLOGY
       Interleukin-2/BLOOD  Intestines/ENZYMOLOGY/PATHOLOGY  Lymph
       Nodes/ENZYMOLOGY/PATHOLOGY  Lymphocyte Transformation
       Retroviridae/GROWTH & DEVELOPMENT/*ISOLATION & PURIF/  PATHOGENICITY
       Retroviridae Infections/IMMUNOLOGY/PATHOLOGY/*VETERINARY/VIROLOGY
       Reverse Transcriptase/BLOOD  RNA, Messenger/ANALYSIS  RNA,
       Viral/ANALYSIS  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).