Document 0825
 DOCN  M9590825
 TI    Adoptive transfer of lymphocytes sensitized to the major surface
       glycoprotein of Pneumocystis carinii confers protection in the rat.
 DT    9509
 AU    Theus SA; Andrews RP; Steele P; Walzer PD; Research Service, Veterans
       Affairs Medical Center, Cincinnati, OH; 45220, USA.
 SO    J Clin Invest. 1995 Jun;95(6):2587-93. Unique Identifier : AIDSLINE
       MED/95286822
 AB    Pneumocystis carinii is a major opportunistic pathogen and a leading
       cause of morbidity in patients with AIDS. CD4+ cells have been shown to
       be important in host defenses against P. carinii, but the antigen(s)
       involved with this response have not been identified. We undertook the
       present study to determine whether the major surface glycoprotein (MSG)
       of P. carinii contains epitopes that can elicit a protective cellular
       immune response. Spleen cells and purified CD4+ cells isolated from
       Lewis rats, pulsed 1-4 d with MSG, and injected into
       corticosteroid-treated Lewis rats with pneumocystosis resulted in
       significant reduction in the P. carinii burden, as judged by organism
       quantitation and lung histology. The protective response demonstrated by
       the donor cells was dependent on previous exposure to P. carinii, cell
       concentration, and time of incubation with MSG. In addition,
       reconstitution with MSG-specific CD4+ cells resulted in an early
       hyperinflammatory response within the lungs of these animals with a high
       percentage of mortality. Thus, in this model, MSG can elicit an immune
       response mediated by CD4+ cells, which has a harmful as well as helpful
       effect on the host, and these responses occur despite the presence of
       corticosteroids.
 DE    Animal  Antigens, Fungal/*IMMUNOLOGY  Fungal Proteins/*IMMUNOLOGY
       Immunity, Cellular  Immunization, Passive  Immunosuppression  Male
       Membrane Glycoproteins/*IMMUNOLOGY  Pneumocystis carinii/*IMMUNOLOGY
       Pneumonia, Pneumocystis carinii/*IMMUNOLOGY/THERAPY  Rats  Rats, Inbred
       Lew  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       T-Lymphocyte Subsets/*IMMUNOLOGY  JOURNAL ARTICLE

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