Document 0828
 DOCN  M9590828
 TI    Detection of HIV-1 RNA in plasma by isothermal amplification (NASBA)
       irrespective of the stage of HIV-1 infection.
 DT    9509
 AU    Zaaijer HL; Kok W; ten Veen JH; Reesink HW; Foolen H; Winkel IN; Huisman
       JG; Cuypers HT; Kievits T; Lelie PN; Central Laboratory of the
       Netherlands Red Cross Blood Transfusion; Service (CLB), Amsterdam.
 SO    J Virol Methods. 1995 Mar;52(1-2):175-81. Unique Identifier : AIDSLINE
       MED/95286739
 AB    Using an experimental assay for isothermal amplification of HIV RNA in
       plasma (NASBA, Organon Teknika, Boxtel, The Netherlands), 70 samples
       from 59 HIV-1-infected persons and 29 samples from 28 uninfected
       volunteer blood donors were tested for the presence of HIV-1 RNA. The
       HIV-1-positive plasma samples were drawn from patients at various stages
       of infection: two samples from persons with signs of acute HIV infection
       (CDC stage I); 29 samples from 25 symptom-free persons (CDC stage II)
       and 39 samples from 32 persons with AIDS-related symptoms (CDC stage
       IV). All samples from HIV-1-infected persons had HIV-1 RNA, irrespective
       of the stage of infection (100% sensitivity). Testing the donor samples
       in duplicate, initially 54/58 samples (93%) tested negative for HIV-1
       RNA. Repeated testing of the 4 samples with inconsistent test results
       showed all samples to be negative (specificity 100%). Detection of HIV-1
       RNA in plasma by isothermal amplification (NASBA) promises to be a
       valuable alternative to the detection of HIV-1 RNA or DNA by the
       polymerase chain reaction.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*DIAGNOSIS  Comparative Study
       DNA Primers  Gene Amplification/*METHODS  Human  HIV
       Seropositivity/BLOOD/*DIAGNOSIS  HIV-1/GENETICS/*ISOLATION & PURIF
       Reproducibility of Results  RNA, Viral/*BLOOD  Sensitivity and
       Specificity  JOURNAL ARTICLE

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