Document 0829
 DOCN  M9590829
 TI    Detection of HIV-1 RNA in plasma and serum samples using the NASBA
       amplification system compared to RNA-PCR.
 DT    9509
 AU    Vandamme AM; Van Dooren S; Kok W; Goubau P; Fransen K; Kievits T; Schmit
       JC; De Clercq E; Desmyter J; Rega Institute for Medical Research,
       Katholieke Universiteit; Leuven, Belgium.
 SO    J Virol Methods. 1995 Mar;52(1-2):121-32. Unique Identifier : AIDSLINE
       MED/95286733
 AB    The presence of HIV-1 RNA in the plasma and serum of European and
       African patients was monitored using RNA-polymerase chain reaction
       (RNA-PCR) and the new isothermal NASBA nucleic acid amplification system
       encompassing a gel-based detection assay (ELGA). Identical RNA
       extraction procedures, provided by the NASBA amplification system, were
       used for both methods. The detection limit for HIV-1 RNA, measured on a
       10-fold dilution series of spiked HIVIIIB in negative plasma, was about
       0.05 CCID50 per test for both methods. Both NASBA and RNA-PCR were more
       sensitive than a p24 assay for the detection of circulating HIV-1 virus
       in blood: 17 of the 34 (50%) p24 antigen-tested seropositives were
       p24-positive while 32 (94%) were positive by NASBA and 30 (88%) by
       RNA-PCR. Among the 45 seropositives, 34 of which were tested for p24
       antigen, 43 (96%) were positive by NASBA and 41 (91%) by RNA-PCR. Almost
       all seropositives had a detectable viral load in 100 microliters plasma.
       Lower viral loads were only encountered in some healthy seropositives
       with a higher CD4 count. There was no cross-reactivity with HIV-2 or
       HIV-I with both the RNA-PCR and NASBA. The extraction method used
       permitted the detection of HIV-1 RNA equally well in serum and in plasma
       with heparin or EDTA.
 DE    Africa/ETHNOLOGY  Algorithms  Base Sequence  Cell Line  Comparative
       Study  Cross Reactions  DNA Primers  Europe  Gene Amplification/METHODS
       Genes, gag  Human  HIV Core Protein p24/BLOOD  HIV
       Seropositivity/*DIAGNOSIS  HIV-1/GENETICS/*ISOLATION & PURIF  Molecular
       Sequence Data  Polymerase Chain Reaction/METHODS  Reproducibility of
       Results  RNA, Viral/*BLOOD  Sensitivity and Specificity  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).