Document 0853
 DOCN  M9590853
 TI    Experimental hypersensitivity pneumonitis: effect of Thy1.2+ and CD8+
       cell depletion.
 DT    9509
 AU    Schuyler M; Gott K; Edwards B; Nikula KJ; Department of Medicine,
       Albuquerque VA Medical Center, University; of New Mexico School of
       Medicine.
 SO    Am J Respir Crit Care Med. 1995 Jun;151(6):1834-42. Unique Identifier :
       AIDSLINE MED/95285036
 AB    We previously demonstrated that recipient CD4+ cells are necessary for
       expression of adoptive murine experimental hypersensitivity pneumonitis
       (EHP). In contrast, the acute inflammatory response to intratracheal
       (i.t.) administration of Micropolyspora faeni (direct EHP) is not CD4+
       cell dependent (Am. J. Respir. Crit. Care Med. 1994;149:1286-1294). To
       further characterize the cells responsible for development of pulmonary
       inflammation in recipient animals, we depleted recipients of either
       Thy1.2+ or CD8+ cells before transfer of M. faeni-sensitized cultured
       C3H/HeJ spleen cells and i.t. challenge with M. faeni. We used the same
       depletion technique to determine the contribution of these cells to the
       pulmonary response to i.t. M. faeni in animals that did not receive
       cultured cells (direct EHP). The nature and extent of pulmonary
       inflammatory changes in these animals were assayed either 4 d after i.t.
       challenge with M. faeni in adoptive EHP or 2 d after i.t. challenge with
       M. faeni in direct EHP. We also tested the hypothesis that our
       previously demonstrated ablation of adoptive EHP caused by
       administration of anti-CD4 antibody was due to depletion of recipient
       CD4+ cells by allowing recovery of recipient CD4+ cells of
       anti-CD4-treated animals before i.t. challenge. In addition, we allowed
       Thy1.2+ cell recovery of anti-Thy1.2-treated animals before i.t.
       challenge. Cultured M. faeni-sensitized spleen cells could adoptively
       transfer EHP to animals treated with an irrelevant antibody or PBS.
       Depletion of Thy1.2+ but not CD8+ cells ablated the ability of recipient
       animals to express adoptive EHP. Direct EHP was not affected by
       depletion of Thy1.2+ or CD8+ cells.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Alveolitis, Extrinsic Allergic/*IMMUNOLOGY  Animal  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY
       Immunotherapy, Adoptive  Lymphocyte Depletion  Male  Mice  Mice, Inbred
       BALB C  Mice, Inbred C3H  Micromonosporaceae/IMMUNOLOGY  Support, U.S.
       Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/*IMMUNOLOGY  JOURNAL ARTICLE

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