Document 0881
 DOCN  M9590881
 TI    Cyclohexylamine inhibits the adhesion of lymphocytic cells to human
       syncytiotrophoblast.
 DT    9509
 AU    Douglas GC; Hu J; Thirkill TL; Hovanes K; Fuller M; King BF; Department
       of Cell Biology and Human Anatomy, School of Medicine,; University of
       California, Davis 95616-8643, USA.
 SO    Biochim Biophys Acta. 1995 May 12;1266(3):229-34. Unique Identifier :
       AIDSLINE MED/95284136
 AB    We have previously shown that lymphocytic cells adhere to cultured
       syncytiotrophoblast and that this may be important in the
       lymphocyte-mediated infection of trophoblast with the human
       immunodeficiency virus (HIV). During the course of studies aimed at
       investigating the role of cell surface carbohydrates in adhesion, it was
       discovered that a contaminant of commercial fucose-1-phosphate,
       dicyclohexylamine, inhibited MOLT-trophoblast adhesion.
       Dicyclohexylamine and the related compounds, cyclohexylamine and
       hexylamine, inhibited adhesion in a dose-responsive manner with
       half-maximal inhibition seen at about 4 mM. While the pressor effects of
       cyclohexylamine, the principal metabolite of cyclamate, are well known,
       this is the first report of an effect of this and related compounds on
       cell adhesion activity. The inhibitory effect was reversible and, at
       concentrations less than 25 mM, did not result in loss of cell
       viability. Several possible mechanisms of action of cyclohexylamine were
       examined in an attempt to explain the effect on adhesion. No evidence
       was found to suggest that the effects of cyclohexylamine were due to
       inhibition of polyamine synthesis, increase in intracellular Ca2+
       concentration or to a lysosomotropic effect. The concentrations of
       cyclohexylamine used are within the range of plasma concentrations
       attainable in humans, raising the possibility that the in vitro effects
       described here may also occur in vivo. The results also suggest that
       caution should be used in the interpretation of results obtained from
       experiments where cell adhesion is blocked using exogenous
       monosaccharides that are in the form of dicyclohexylammonium salts.
       Appropriate controls must be included or, if possible, sodium, potassium
       or barium salts should be chosen.
 DE    Cell Adhesion/*DRUG EFFECTS  Clone Cells
       Cyclohexylamines/*CHEMISTRY/*PHARMACOLOGY  Dose-Response Relationship,
       Drug  Fructosephosphates/PHARMACOLOGY  Fucose/*ANALOGS &
       DERIVATIVES/CHEMISTRY  Hexosephosphates/*CHEMISTRY  Human
       Lymphocytes/DRUG EFFECTS  Support, U.S. Gov't, P.H.S.
       Trophoblast/CYTOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).