Document 0888
 DOCN  M9590888
 TI    Interleukin-8 induces its own production in CD4+ T lymphocytes: a
       process regulated by interleukin 10.
 DT    9509
 AU    Gesser B; Deleuran B; Lund M; Vestergard C; Lohse N; Deleuran M; Jensen
       SL; Pedersen SS; Thestrup-Pedersen K; Larsen CG; Department of
       Dermatology, Marselisborg Hospital, University of; Aarhus, Denmark.
 SO    Biochem Biophys Res Commun. 1995 May 25;210(3):660-9. Unique Identifier
       : AIDSLINE MED/95283523
 AB    The neutrophil and T cell chemotactic factor interleukin 8 (IL-8) is
       believed to play a pathophysiological role in the development of various
       inflammatory disorders. So far no other effects of IL-8 on T cells have
       been observed. We observed that purified CD4+ T cells in particular, but
       also CD8+ T cells, spontaneously synthesize IL-8 mRNA and secrete IL-8
       protein. The culture supernatants of CD4+ T cells contained T cell
       chemotactic activity as well as IL-8 protein. In addition, we confirmed
       the ability of CD4+ T cells to produce IL-8 by double immunofluorescence
       staining and by the demonstration of IL-8 mRNA expression. Further, IL-8
       induced its own production in CD4+ T cells, while its synthesis by CD8+
       T cells was low and not always auto-stimulatory. Both the spontaneous,
       as well as the IL-8 induced IL-8 production, could be inhibited in the
       presence of human interleukin 10 (100 ng/ml). This observation suggests
       that IL-10 plays a homeostatic role in regulating the IL-8 circuit in
       CD4+ T cells.
 DE    Autoradiography  Base Sequence  Cells, Cultured  Chemotaxis, Leukocyte
       CD4-Positive T-Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/DRUG EFFECTS/*IMMUNOLOGY  DNA Primers  Electrophoresis,
       Gel, Two-Dimensional  Electrophoresis, Polyacrylamide Gel  Fluorescent
       Antibody Technique  Gene Expression/DRUG EFFECTS  Gene Expression
       Regulation  Human  Interleukin-10/*PHARMACOLOGY
       Interleukin-8/*BIOSYNTHESIS/ISOLATION & PURIF/*PHARMACOLOGY
       Methionine/METABOLISM  Molecular Sequence Data  Polymerase Chain
       Reaction  Recombinant Proteins/PHARMACOLOGY  RNA, Messenger/BIOSYNTHESIS
       Sulfur Radioisotopes  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).